POLYMER SUPPORT OLIGONUCLEOTIDE SYNTHESIS .18. USE OF BETA-CYANOETHYL-N,N-DIALKYLAMINO-/N-MORPHOLINO PHOSPHORAMIDITE OF DEOXYNUCLEOSIDES FOR THE SYNTHESIS OF DNA FRAGMENTS SIMPLIFYING DEPROTECTION AND ISOLATION OF THE FINAL PRODUCT
POLYMER SUPPORT OLIGONUCLEOTIDE SYNTHESIS .18. USE OF BETA-CYANOETHYL-N,N-DIALKYLAMINO-/N-MORPHOLINO PHOSPHORAMIDITE OF DEOXYNUCLEOSIDES FOR THE SYNTHESIS OF DNA FRAGMENTS SIMPLIFYING DEPROTECTION AND ISOLATION OF THE FINAL PRODUCT
复制标题
DOI：
10.1093/nar/12.11.4539
复制
发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
KOSTER, H
中科院分区：
文献类型：
--
作者：
SINHA, ND;BIERNAT, J;KOSTER, H
Various 5′-0-N-protected deoxynucleoside-3′-0-β-cyanoethyl-N,N-dialkylamino-/N-morpholinophosphoramidites were prepared from β-cyanoethyl monochlorophosphoramidites of N,N-dimethylamine, N,N-diisopropylamine and N-morpholine. These active deoxynucleoside phosphites have successfully been used for oligodeoxy-nucleotide synthessis on controlled pore glass as polymer support and are very suitable for automated DNA-synthesis due to their stability in solution. The intermediate dichloro-β-cyanoethoxy-phosphine can easily be prepared free from any PCl3contamination. The active monomers obtained from β-cyanoethyl monochloro N,N-diisopropylaminophosphoramidites are favoured. Cleavage of the oligonucleotide chain from the polymer support, N-deacylationanddeprotection of β-cyanoethyl group from the phosphate triester moiety can be performed inone stepwith concentrated aqueous ammonia. Mixed oligodeoxynucleotides are characterized by the sequencing method of Maxam and Gilbert.
