Glial Glutamate Transporters Mediate a Functional Metabolic Crosstalk between Neurons and Astrocytes in the Mouse Developing Cortex
Glial Glutamate Transporters Mediate a Functional Metabolic Crosstalk between Neurons and Astrocytes in the Mouse Developing Cortex
复制标题
DOI：
10.1016/s0896-6273(02)01170-4
复制
发表时间：
2003-01
期刊：
影响因子：
16.2
通讯作者：
Brigitte Voutsinos-Porche;G. Bonvento;Kohichi Tanaka;P. Steiner;E. Welker;J. Chatton;P. Magistretti;L. Pellerin
中科院分区：
文献类型：
--
作者：
Brigitte Voutsinos-Porche;G. Bonvento;Kohichi Tanaka;P. Steiner;E. Welker;J. Chatton;P. Magistretti;L. Pellerin
神经元-胶质细胞的相互作用对突触功能至关重要，而胶质细胞谷氨酸（再）摄取在突触能突触中起着关键作用。在敲除小鼠中，对于胶质谷氨酸转运蛋白GLAST或GLT-1，对突触激活的经典代谢反应（即，葡萄糖利用的增强）在晶须激活后的躯体感觉桶皮质中的早期功能阶段降低。体外研究表明，神经胶质细胞谷氨酸转运是触发葡萄糖利用率提高的关键步骤，也是通过涉及细胞内Na+浓度变化的机制从星形胶质细胞释放乳酸的关键步骤。这些数据表明，在发育中的皮层神经元和星形胶质细胞之间发生代谢串扰，这将受到突触活动的调节，并由胶质细胞谷氨酸转运蛋白介导。
Neuron-glia interactions are essential for synaptic function, and glial glutamate (re)uptake plays a key role at glutamatergic synapses. In knockout mice, for either glial glutamate transporters, GLAST or GLT-1, a classical metabolic response to synaptic activation (i.e., enhancement of glucose utilization) is decreased at an early functional stage in the somatosensory barrel cortex following activation of whiskers. Investigation in vitro demonstrates that glial glutamate transport represents a critical step for triggering enhanced glucose utilization, but also lactate release from astrocytes through a mechanism involving changes in intracellular Na+concentration. These data suggest that a metabolic crosstalk takes place between neurons and astrocytes in the developing cortex, which would be regulated by synaptic activity and mediated by glial glutamate transporters.