The Arabidopsis transcription factor AINTEGUMENTA orchestrates patterning genes and auxin signaling in the establishment of floral growth and form
The Arabidopsis transcription factor AINTEGUMENTA orchestrates patterning genes and auxin signaling in the establishment of floral growth and form
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DOI：
10.1111/tpj.14769
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发表时间：
2020-05-05
期刊：
影响因子：
7.2
通讯作者：
Loraine, Ann E.
中科院分区：
文献类型：
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作者：
Krizek, Beth A.;Blakley, Ivory C.;Loraine, Ann E.
了解花是如何形成的是植物生物学中的一个重要问题，因为人类食物供应取决于花和种子的生产。花发育还为了解器官发生过程中细胞分裂、扩张和分化如何协调提供了一个极好的模型。在模式植物拟南芥中，花器官发生需要 AINTEGUMENTA (ANT) 和 AINTEGUMENTA-LIKE 6 (AIL6)/PLETHORA 3 (PLT3)，它们是拟南芥 AINTEGUMENTA-LIKE/PLETHORA (AIL/PLT) 转录因子家族的两个成员。 ANT 和 AIL6/PLT3 共同调节花器官发生的各个方面，包括花器官的起始、生长、身份规范和模式。此前，我们使用 RNA-Seq 鉴定了 ant ail6 突变花中数千个表达中断的基因，表明 ANT 和 AIL6/PLT3 影响着巨大的转录网络。然而，ANT 和 AIL6/PLT3 在花中的直接下游靶标尚不清楚。为了确定 ANT 调节的直接靶标，我们进行了 RNA 测序时程实验，在带有 ANT-糖皮质激素受体融合构建体的转基因植物中诱导 ANT 活性。此外，我们还进行了 ChIP-Seq 实验，鉴定了正在发育的花朵中的 ANT 结合位点。这些实验根据 ANT 结合位点的接近程度以及对 ANT 反应的差异表达，确定了 200 个潜在的 ANT 靶基因。这 200 个候选靶基因涉及极性规范、花器官发育、分生组织发育和生长素信号传导等功能。此外，我们还发现了几个与侧器官生长相关的基因，这些基因可能介导 ANT 在器官大小控制中的作用。这些结果通过将 ANT 与以前未知的调控目标联系起来，揭示了 ANT 转录网络的新特征。
Understanding how flowers form is an important problem in plant biology, as human food supply depends on flower and seed production. Flower development also provides an excellent model for understanding how cell division, expansion and differentiation are coordinated during organogenesis. In the model plant Arabidopsis thaliana, floral organogenesis requires AINTEGUMENTA (ANT) and AINTEGUMENTA-LIKE 6 (AIL6)/PLETHORA 3 (PLT3), two members of the Arabidopsis AINTEGUMENTA-LIKE/PLETHORA (AIL/PLT) transcription factor family. Together, ANT and AIL6/PLT3 regulate aspects of floral organogenesis, including floral organ initiation, growth, identity specification and patterning. Previously, we used RNA-Seq to identify thousands of genes with disrupted expression in ant ail6 mutant flowers, indicating that ANT and AIL6/PLT3 influence a vast transcriptional network. The immediate downstream targets of ANT and AIL6/PLT3 in flowers are unknown, however. To identify direct targets of ANT regulation, we performed an RNA-Seq time-course experiment in which we induced ANT activity in transgenic plants bearing an ANT-glucocorticoid receptor fusion construct. In addition, we performed a ChIP-Seq experiment that identified ANT binding sites in developing flowers. These experiments identified 200 potential ANT target genes based on their proximity to ANT binding sites and differential expression in response to ANT. These 200 candidate target genes were involved in functions such as polarity specification, floral organ development, meristem development and auxin signaling. In addition, we identified several genes associated with lateral organ growth that may mediate the role of ANT in organ size control. These results reveal new features of the ANT transcriptional network by linking ANT to previously unknown regulatory targets.