Charge Balancing of Model Gold-Nanoparticle-Peptide Conjugates Controlled by the Peptide’s Net Charge and the Ligand to Nanoparticle Ratio
Charge Balancing of Model Gold-Nanoparticle-Peptide Conjugates Controlled by the Peptide’s Net Charge and the Ligand to Nanoparticle Ratio
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DOI：
10.1021/jp501489t
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发表时间：
2014-05
影响因子：
3.7
通讯作者：
L. Gamrad;C. Rehbock;J. Krawinkel;B. Tumursukh;A. Heisterkamp;S. Barcikowski
中科院分区：
文献类型：
--
作者：
L. Gamrad;C. Rehbock;J. Krawinkel;B. Tumursukh;A. Heisterkamp;S. Barcikowski
金纳米颗粒（AuNPs）共价结合到生物分子上，称为生物缀合物，1与药物靶向或生物成像等生物应用高度相关。在这里，生物缀合物的净电荷是影响生物相容性和细胞膜相互作用的一个关键参数。然而，当带负电荷的AuNP与带正电荷的生物分子缀合时，所得的电荷补偿损害了缀合物的稳定性。在这项工作中，激光产生的带负电荷的金纳米粒子表现出裸露的表面被用作一个模型，并分别缀合到细胞穿透肽（CPP）携带不同的正净电荷。双电荷补偿导致两个方案，其中在生物缀合物的等电点的两侧获得稳定的生物缀合物。这些方案可以通过肽的净电荷来控制。通常，增加肽的净电荷产生具有降低的表面覆盖率的稳定的带正电荷的生物缀合物。为了证明兼容...
Gold nanoparticles (AuNPs) covalently bound to biomolecules, termed bioconjugates,1 are highly relevant for biological applications like drug targeting or bioimaging. Here, the net charge of the bioconjugate is one key parameter affecting biocompatibility and cell membrane interaction. However, when negatively charged AuNPs are conjugated to positively charged biomolecules, resulting charge compensation compromises the stability of the conjugates. In this work, laser-generated negatively charged AuNPs exhibiting a bare surface were used as a model and separately conjugated to cell penetrating peptides (CPPs) carrying different positive net charges. Occurring charge compensation leads to two regimes where stable bioconjugates are obtained on both sides of the bioconjugate’s isoelectric point. These regimes can be controlled by the peptide’s net charge. Generally, increasing the peptide’s net charges yields stable positively charged bioconjugates with decreased surface coverages. To demonstrate the compatib...