Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice.
Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice.
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DOI：
10.1007/978-1-4939-8691-0_18
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Stroud MJ;Fang X;Veevers J;Chen J
核骨架和细胞骨架（LINC）复合物的连接物介导细胞核和细胞质之间的胞内串扰。在横纹肌中，LINC复合体为肌细胞核提供结构支持，并在调节基因表达和机械转导中发挥重要作用。广泛的心脏和骨骼肌病与LINC复合物蛋白的突变有关。利用组织特异性敲除和突变小鼠模型的研究揭示了LINC复合物在横纹肌中的作用的重要见解。在本章中，我们描述了几种可行的方法来生成横纹肌特异性基因敲除和突变小鼠模型，以研究LINC复合蛋白在心脏和骨骼肌中的功能。实验程序用于表型和分析的LINC复杂敲除小鼠也进行了描述。
The linker of nucleoskeleton and cytoskeleton (LINC) complex mediates intracellular cross talk between the nucleus and the cytoplasm. In striated muscle, the LINC complex provides structural support to the myocyte nucleus and plays an essential role in regulating gene expression and mechanotransduction. A wide range of cardiac and skeletal myopathies have been linked to mutations in LINC complex proteins. Studies utilizing tissue-specific knockout and mutant mouse models have revealed important insights into the roles of the LINC complex in striated muscle. In this chapter, we describe several feasible approaches for generating striated muscle-specific gene knockout and mutant mouse models to study LINC complex protein function in cardiac and skeletal muscle. The experimental procedures used for phenotyping and analysis of LINC complex knockout mice are also described.