Cre Recombinase
Cre Recombinase
复制标题
DOI：
10.1128/microbiolspec.mdna3-0014-2014
复制
发表时间：
2015-02-01
影响因子：
3.7
通讯作者：
van Duyne, Gregory D.
中科院分区：
文献类型：
--
作者：
van Duyne, Gregory D.
利用Cre重组酶进行转基因的条件诱变和将DNA磁带插入真核生物染色体是广泛的。自近30年前Cre首次在酵母和哺乳动物细胞中发挥作用以来，Cre- loxp系统在体内和体外的大量应用已被报道，此外，Cre- loxp系统在了解酪氨酸重组酶家族位点特异性重组酶的重组机制方面也发挥了重要作用。该系统的简单性，只需要一个重组酶和短重组序列在各种情况下具有强大的活性，是这两种情况下的一个重要因素。这篇综述讨论了自《移动DNA II》出版以来，过去12年来在Cre重组酶领域取得的进展。重点是那些最近的贡献，为反应提供了新的机制见解。还讨论了Cre和/或loxP序列的修饰，这些修饰导致了基因组工程应用的改进。
The use of Cre recombinase to carry out conditional mutagenesis of transgenes and insert DNA cassettes into eukaryotic chromosomes is widespread. In addition to the numerous in vivo and in vitro applications that have been reported since Cre was first shown to function in yeast and mammalian cells nearly 30 years ago, the Cre-loxP system has also played an important role in understanding the mechanism of recombination by the tyrosine recombinase family of site-specific recombinases. The simplicity of this system, requiring only a single recombinase enzyme and short recombination sequences for robust activity in a variety of contexts, has been an important factor in both cases. This review discusses advances in the Cre recombinase field that have occurred over the past 12 years since the publication of Mobile DNA II. The focus is on those recent contributions that have provided new mechanistic insights into the reaction. Also discussed are modifications of Cre and/or the loxP sequence that have led to improvements in genome engineering applications.