Erosion of dosage compensation impacts human iPSC disease modeling.
Erosion of dosage compensation impacts human iPSC disease modeling.
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DOI：
10.1016/j.stem.2012.02.014
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发表时间：
2012-05-04
期刊：
影响因子：
23.9
通讯作者：
Eggan, Kevin
中科院分区：
文献类型：
--
作者：
Mekhoubad, Shila;Bock, Christoph;de Boer, A. Sophie;Kiskinis, Evangelos;Meissner, Alexander;Eggan, Kevin
Although distinct human induced pluripotent stem cell (hiPSC) lines can display considerable epigenetic variation, it has been unclear if such variability impacts their utility for disease modeling. Here, we show that although low passage female hiPSCs retain the inactive X-chromosome of the somatic cell they are derived from, over time in culture they undergo an “erosion” of X-chromosome inactivation (XCI). This erosion of XCI is characterized by loss of XIST expression and foci of H3-K27-trimethylation, as well as transcriptional de-repression of genes on the inactive X that cannot be reversed by either differentiation or further reprogramming. We specifically demonstrate that erosion of XCI has a significant impact on the use of female hiPSCs for modeling Lesch-Nyhan syndrome. However, our finding that most genes subject to XCI are de-repressed by this erosion of XCI suggests that it should be a significant consideration when selecting hiPSC lines for modeling any disease.
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