Dental Stem Cell Therapy with Calcium Hydroxide in Dental Pulp Capping
Dental Stem Cell Therapy with Calcium Hydroxide in Dental Pulp Capping
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DOI：
10.1089/ten.tea.2009.0054
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发表时间：
2010-06-01
影响因子：
4.1
通讯作者：
Choung, Pill-Hoon
中科院分区：
文献类型：
--
作者：
Ji, Young-Min;Jeon, Soung Hoo;Choung, Pill-Hoon
氢氧化钙直接盖髓在现代口腔临床中已得到广泛而稳定的应用。由于已知其具有诱导硬组织修复的潜力，因此已将该化学品应用于暴露的牙髓，并且预计硬组织将在牙髓上方再生。在暴露牙髓的修复过程中，由于广泛损伤而丢失的初级成牙本质细胞被新分化的成牙本质细胞样细胞所取代。已知该过程遵循祖细胞的增殖、迁移和分化的顺序步骤。本研究将探讨氢氧化钙与从比格犬未成熟牙组织中获得的出生后牙干细胞的招募、增殖和矿化之间的关系。免疫细胞化学染色和逆转录聚合酶链反应被用来确定假定的干细胞标志物。免疫印迹分析，伤口愈合试验，细胞迁移试验，茜素红染色用于评估的增殖，迁移和矿化能力的氢氧化钙处理的干细胞。作为体内研究，氢氧化钙和自体牙髓干细胞（DPSC）的组合应用于治疗比格犬的前磨牙和磨牙上的有意创建的牙齿缺损，以观察牙本质再生。体外扩增的DPSC和牙周膜干细胞表达间充质干细胞标志物STRO-1和CD 146。可见，氢氧化钙可促进DPSC和牙周膜干细胞的募集、迁移、增殖和矿化。这些结果对于DPSC的未来可用性是有价值的，DPSC最近被关注为牙齿损伤后牙本质再生的干细胞库，以及阐明氢氧化钙在盖髓治疗中的作用。
Calcium hydroxide has been extensively and steadily used for direct pulp capping in modern clinical dentistry. As it was known to have potential to induce hard tissue repair, this chemical has been applied to the exposed dental pulp and the hard tissue is expected to be regenerated above the pulp. During the reparative process of exposed pulp, primary odontoblasts that were lost as a result of extensive damage are replaced with newly differentiated odontoblast-like cells. This process is known to follow the sequential steps of proliferation, migration, and differentiation of progenitor cells. This research will examine the relationship between calcium hydroxide and the recruitment, proliferation, and mineralization of postnatal dental stem cells, obtained from an immature dental tissue of beagle dogs. Immunocytochemical staining and reverse transcriptase-polymerase chain reaction were used to identify the putative stem cell markers. Immunoblot analysis, wound healing assay, cell migration assay, and alizarin red staining were used to evaluate proliferation, migration, and mineralization capacity of the calcium hydroxide-treated stem cells. As an in vivo study, a combination of calcium hydroxide and autologous dental pulp stem cells (DPSCs) was applied for the treatment of intentionally created tooth defects on the premolars and the molars in beagle dogs to observe dentin regeneration. Ex vivo expanded DPSCs and periodontal ligament stem cells expressed STRO-1 and CD146, the mesenchymal stem cell markers. It was evident that calcium hydroxide increased recruitment, migration, proliferation, and mineralization of the DPSCs and periodontal ligament stem cells. Such results are valuable for future availability of DPSCs, which are recently focused as the stem cell reservoir for regeneration of dentin upon tooth injury, as well as for elucidation of the role of calcium hydroxide in pulp capping therapy.