Evaluation of reference genes and expression of key genes involved in the isoprenoid metabolic pathway of rice leaves after infection by the Southern rice black-streaked dwarf virus
Evaluation of reference genes and expression of key genes involved in the isoprenoid metabolic pathway of rice leaves after infection by the Southern rice black-streaked dwarf virus
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DOI：
10.1007/s11033-019-04841-4
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发表时间：
2019-04
影响因子：
2.8
通讯作者：
Kui Hu;Lin Qiu;W. Ding;Hua-liang He;Youzhi Li
中科院分区：
文献类型：
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作者：
Kui Hu;Lin Qiu;W. Ding;Hua-liang He;Youzhi Li
南方水稻黑条矮缩病毒（Southern rice black-streaked dwarf virus，SRBSDV）是一种新的斐济病毒，对东亚地区的水稻生产构成了重大威胁。对SRBSDV感染水稻植株基因表达的分析可能揭示病毒及其载体与水稻相互作用的分子基础。准确的qRT-PCR分析需要可靠的参考基因。然而，没有可靠的，有效的参考基因检测SRBSDV感染的水稻植株中的基因表达迄今已被确定。我们研究了8个候选参考基因的表达在SRBSDV感染，健康，水稻植株在不同的时间点接种病毒后的叶片。我们使用了四种专用算法，geNorm，BestKeeper，Normalization和Referral，来评估这些候选基因的性能。参考结果表明，18 S、EF 1 α和UBQ 10是比较合适的参考基因。此外，我们还利用这三个参考基因分析了水稻叶片感染SRBSDV后类异戊二烯代谢途径关键基因的表达。结果表明，SRBSDV可能抑制了水稻植株挥发物的产生，从而增加了病原菌传播的可能性。
The Southern rice black-streaked dwarf virus (SRBSDV), a novelFijivirus, poses a major threat to rice production in East Asia. Analysis of the gene expression of SRBSDV-infected rice plants may reveal the molecular basis of interactions between the virus, its vector and rice plants. Reliable reference genes are required for accurate qRT-PCR analysis. However, no reliable, valid reference genes for examining gene expression in SRBSDV-infected rice plants have so far been identified. We examined the expression of eight candidate reference genes in the leaves of SRBSDV-infected, and healthy, rice plants at different points in time after virus inoculation. We used four dedicated algorithms, geNorm, BestKeeper, NormFinder and RefFinder, to evaluate the performance of these candidate genes. The RefFinder results indicate that18S,EF1αandUBQ10are suitable reference genes. In addition, we used these three reference genes to analyze the expression of key genes involved in the isoprenoid metabolic pathway in rice leaves after infection by SRBSDV. The results of this analysis reveal that SRBSDV may suppress the production of the rice plant volatiles that attract natural enemies of its vectorSogatella furcifera, thereby increasing the likelihood of pathogen transmission.