Fine mapping of the epistatic suppressor gene (esp) of a recessive genic male sterility in rapeseed (Brassica napus L.).
Fine mapping of the epistatic suppressor gene (esp) of a recessive genic male sterility in rapeseed (Brassica napus L.).
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DOI：
10.1139/g09-049
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发表时间：
2009-08
期刊：
影响因子：
3.1
通讯作者：
Zhenghua Xu;Yanzhou Xie;Dengfeng Hong;Ping-wu Liu;Guangsheng Yang
中科院分区：
文献类型：
--
作者：
Zhenghua Xu;Yanzhou Xie;Dengfeng Hong;Ping-wu Liu;Guangsheng Yang
甘蓝型油菜隐性核不育系9012AB是由甘蓝型油菜自发突变选育而成，在我国油菜杂交种生产中占有重要地位。9012AB的雄性不育性受两对隐性基因ms3、ms4和一对隐性上位抑制基因esp互作控制。本研究的目的是开发与esp基因紧密连锁的基于PCR的标记，并构建围绕esp基因的高分辨率图谱。通过对512个AFLP引物组合的调查，获得了3个紧密连锁的AFLP标记，并成功转化为共显性或显性SCAR标记。此外，通过遗传图谱整合，确定了一个与esp基因相关的共显性SSR标记（Ra2G08）。对于esp基因的精细作图，在2545株植物的大BC1群体中分析这些基于PCR的标记。将esp基因遗传限制在1.03 cM的区域内，SSR标记Ra2G08的限制性片段为0.35 cM，SCAR标记WSC6的限制性片段为0.68 cM。SCAR标记WSC5与目的基因共分离。这些结果为超感蛋白的图位克隆奠定了坚实的基础，并将有助于RGMS系及其临时保持系的选择。
9012AB, a recessive genic male sterility (RGMS) line derived from spontaneous mutation in Brassica napus, has been playing an important role in rapeseed hybrid production in China. The male sterility of 9012AB is controlled by two recessive genes (ms3 and ms4) interacting with one recessive epistatic suppressor gene (esp). The objective of this study was to develop PCR-based markers tightly linked to the esp gene and construct a high-resolution map surrounding the esp gene. From the survey of 512 AFLP primer combinations, 3 tightly linked AFLP markers were obtained and successfully converted to codominant or dominant SCAR markers. Furthermore, a codominant SSR marker (Ra2G08) associated with the esp gene was identified through genetic map integration. For fine mapping of the esp gene, these PCR-based markers were analyzed in a large BC1 population of 2545 plants. The esp gene was then genetically restricted to a region of 1.03 cM, 0.35 cM from SSR marker Ra2G08 and 0.68 cM from SCAR marker WSC6. The SCAR marker WSC5 co-segregated with the target gene. These results lay a solid foundation for map-based cloning of esp and will facilitate the selection of RGMS lines and their temporary maintainers.