A 4-ELECTRODE SYSTEM FOR MEASUREMENT OF BILAYER-LIPID MEMBRANE CAPACITANCE
A 4-ELECTRODE SYSTEM FOR MEASUREMENT OF BILAYER-LIPID MEMBRANE CAPACITANCE
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DOI：
10.1088/0957-0233/6/7/028
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发表时间：
1995-07-01
影响因子：
2.4
通讯作者：
FIGASZEWSKI, Z
中科院分区：
文献类型：
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作者：
KALINOWSKI, S;FIGASZEWSKI, Z
介绍了一种用于双分子膜电容测量的四电极电容-周期转换器。电容测量包括用恒定电流对测量的电容进行循环充电和放电，以确定其绝对值。所研究的电容器的电压呈三角形。周期持续时间与输入电容成正比。采用四电极系统进行电容测量，可以大大减小电极和电解液阻抗引起的误差。使用高阻微电极是可能的。该系统使在施加的极化电势下测量电容成为可能；在测量期间，电压围绕该值振荡。这使得测量作为极化电位的函数的膜电容成为可能。给出了电容-周期转换器在计算机控制测量系统中的应用实例。
A four-electrode capacitance-to-period converter designed for capacitance measurements of bilayer lipid membranes is described. The capacitance measurement consists of cyclically charging and discharging the measured capacitance with a constant current, regarding its absolute value. The voltage of the studied capacitor is triangularly shaped. The cycle duration is proportional to the input capacitance. Capacitance measurement with a four-electrode system makes it possible to reduce considerably the errors caused by electrode and electrolyte impedance. It is possible to use high-resistance microelectrodes. The system makes it possible to measure the capacitance at an imposed polarization potential; the voltage oscillates about that value during the measurement. This makes it possible to measure the membrane capacitance as a function of polarization potential. An example is cited of using the capacitance-to-period converter in a computer-controlled measuring system.