Rhythmic and light-inducible appearance of clock-associated pseudo-response regulator protein PRR9 through programmed degradation in the dark in Arabidopsis thaliana
Rhythmic and light-inducible appearance of clock-associated pseudo-response regulator protein PRR9 through programmed degradation in the dark in Arabidopsis thaliana
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DOI：
10.1093/pcp/pcm122
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发表时间：
2007-11-01
影响因子：
4.9
通讯作者：
Mizuno, Takeshi
中科院分区：
文献类型：
--
作者：
Ito, Shogo;Nakamichi, Norihito;Mizuno, Takeshi
在拟南芥中，目前认为伪反应调节蛋白（PSEUDO-RESPONSE REGULATOR， PRR）的一个小家族的成员，包括TOC1 (CAB表达时序1)，协同发挥接近生物钟的作用。在这些PRR成员中，PRR9基因的独特之处在于它的转录不仅每天振荡，而且还以依赖光敏色素的方式被光快速诱导。转录水平上的这些事件对于PRR9的时钟相关功能至关重要。尽管如此，人们对PRR9蛋白产物本身在植物细胞中的表达知之甚少。在这里，我们发现PRR9多肽本身每天都在振荡，并且它们在光照下迅速积累。我们的工作进一步表明，PRR9多肽的存在是通过蛋白酶体介导的程序性降解在黑暗中控制的。
In Arabidopsis thaliana, it is currently believed that the members of a small family of PSEUDO-RESPONSE REGULATOR (PRR) proteins, including TOC1 (TIMING OF CAB EXPRESSION 1), coordinately play roles close to the circadian clock. Among these PRR members, the PRR9 gene is unique in that not only does its transcription oscillate diurnally, but it is also rapidly induced by light in a manner dependent on phytochromes. These events at the level of transcription must be crucial for the clock-associated functions of PRR9. Nonetheless, little is known about the expression of the PRR9 protein product itself in plant cells. Here, we show that PRR9 polypeptides themselves oscillate diurnally, and that they accumulate rapidly in response to light. Our work further suggests that the presence of PRR9 polypeptides is controlled through proteasome-mediated programmed degradation in the dark.