Detection of EBV in nasopharyngeal carcinoma by quantum dot fluorescent in situ hybridization
Detection of EBV in nasopharyngeal carcinoma by quantum dot fluorescent in situ hybridization
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DOI：
10.1016/j.yexmp.2010.09.004
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发表时间：
2010-12-01
影响因子：
3.6
通讯作者：
Xia, He-shun
中科院分区：
文献类型：
--
作者：
Chen, Hong-lei;Peng, Jun;Xia, He-shun
目的：鼻咽癌是东南亚常见的癌症，常与EB病毒感染相关。本研究的主要目的是通过探索量子点在FISH检测中的应用来改进EBV的检测方法，并将基于QD的FISH与常规ISH进行比较。材料和方法：回顾35例鼻咽癌患者的活检标本作为石蜡包埋的组织块。QD-FISH是用链霉亲和素标记的量子点间接标记生物素标记的EBER寡核苷酸探针检测EBV编码的小RNA(EBER)的方法。结果：35例鼻咽癌均为非角化性癌，其中分化型7例，未分化型28例。QD-FISH和ISH检测EBER阳性信号分别为91.43%(32/35)和80%(28/35)。两种方法检测EBER阳性病例数无显著差异。对于EBER状态，QD-FISH和ISH之间有中等一致性(kappa=0.55)。4例EBV原位杂交阴性病例经QD-FISH检测均呈EBER阳性信号。结论：EBV与鼻咽癌的发病密切相关。QD-FISH是一种新的有效的EBER检测方法，与传统的ISH有一定的一致性。(C)2010 Elsevier Inc.保留所有权利。
Aims: Nasopharyngeal carcinoma (NPC) is a common cancer in Southeast Asia and is frequently associated with Epstein-Barr virus (EBV) infection. The primary aim of this study was to improve the method of EBV detection by exploring quantum dots in FISH detection, and compare QD-based FISH with conventional ISH.Materials and methods: Biopsy specimens were retrospectively retrieved from 35 NPC patients as paraffin-embedded tissue blocks. QD-FISH was developed to detect the presence of EBV encoded small RNA (EBER) using biotin-labeled EBER oligonucleotide probe indirectly labeled with streptavidin-conjugated quantum dots. Conventional ISH was also performed using a commercial kit to assess concordance between the two methods.Results: All the 35 NPC cases were nonkeratinizing carcinoma (7 differentiated and 28 undifferentiated subtypes). EBER-positive signals were detected in 91.43% (32/35) and 80% (28/35) cases by QD-FISH and ISH, respectively. There was no significant difference in the number of EBER-positive cases by the two methods. A moderate concordance was found between QD-FISH and ISH for EBER status (kappa = 0.55). Four EBER-negative cases by ISH showed EBER-positive signals when detected by QD-FISH.Conclusions: EBV is closely associated with NPC in Chinese patients. QD-FISH is a novel effective method for EBER detection, and has a moderate concordance with conventional ISH. (C) 2010 Elsevier Inc. All rights reserved.