Clinical available circulating tumor cell assay based on tetra(4-aminophenyl) porphyrin mediated reduced graphene oxide field effect transistor
Clinical available circulating tumor cell assay based on tetra(4-aminophenyl) porphyrin mediated reduced graphene oxide field effect transistor
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基于四（4-氨基苯基）卟啉介导的还原氧化石墨烯场效应晶体管的临床可用循环肿瘤细胞测定
DOI：
10.1016/j.electacta.2019.05.039
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发表时间：
2019-08-01
影响因子：
6.6
通讯作者：
Jia, Yunfang
中科院分区：
文献类型：
--
作者：
Hu, Shihui;Wang, Zhongrong;Jia, Yunfang
循环肿瘤细胞（CTC）检测是一种新兴的肿瘤诊断“液体活检”技术，一种操作简单、技术先进的CTC传感器是临床医学实验室的迫切需要。采用四（4-氨基苯基）卟啉介导的还原氧化石墨烯作为通道材料，基于适体（AS1411）功能化石墨烯场效应晶体管（GFET）的临床样品CTC检测无标签固态传感器。研究对象是49例乳腺癌患者和5例健康人的抗凝血临床样本和缓冲液中A549、MDA-MB-231、HeLa和HUVEC（作为对照）的ctc和EDTA抗凝血全血样本。A549 （R-2 = 0.97）、MDA-MB-231 （R-2 = 0.96）和HeLa （R-2 = 0.98）对ctc在10 ~ 10(6)个细胞/ml范围内的敏感性分别为3.14、3.32、2.60%/log(10)（cells/ml）。同时，对ctc的选择性也得到了证实，对HUVEC的敏感性和R-2分别为-0.13%/log(10)（cells/ml）和0.50。此外，全血干扰使传感范围变窄。最后，发现它们在临床血液样本分级中的应用与这些患者的平行诊断结论一致，也可以识别腺纤维瘤样本和化疗效果。本文提出的基于GFET的CTC传感器可实现适体特异性的直接电子CTC检测，为癌症临床检测提供了一种新的分析技术。(C) 2019 Elsevier Ltd.版权所有。
Circulating tumor cell (CTC) assay is one of the emerging "liquid biopsy" for cancer's diagnostic, an easy-to-operate and state-of-the-art CTC sensor is the imperative need of clinical medical laboratory. A labelfree and solid-state sensor for clinical sample's CTC detection based on an aptamer (AS1411) functionalized graphene field effect transistor (GFET) by using tetra(4-aminophenyl) porphyrin mediated reduced graphene oxide as the channel material. Objects are CTCs of A549, MDA-MB-231, HeLa and HUVEC (as a control) in buffer solutions and EDTA anti-coagulant whole blood samples, as well as the anti-coagulant clinical blood samples from 49 breast cancer patients and 5 healthy persons. The sensitivities for CTCs in the range of 10 - 10(6) cells/ml are 3.14, 3.32, 2.60%/log(10)(cells/ml) for A549 (R-2 = 0.97), MDA-MB-231 (R-2 = 0.96) and HeLa (R-2 = 0.98), respectively. Meanwhile, the selectivity for CTCs is also confirmed by the low sensitivity and R-2 for HUVEC which are -0.13%/log(10)(cells/ml) and 0.50. Furthermore, whole blood interference is found to make the sensing range narrow. At last, their applications in grading clinical blood samples are found to be in agreements with the parallel diagnostic conclusions of these patients, samples of adenofibroma and effect of chemotherapy can also be identified. The aptamer specific, directly electronic CTC detection is accomplished by the proposed GFET based CTC sensor, it can provide a novel analytical technique for cancers' clinical assay. (C) 2019 Elsevier Ltd. All rights reserved.