An LC-MS-Based Metabolomics Approach for Exploring Urinary Metabolome Modifications after Cocoa Consumption
An LC-MS-Based Metabolomics Approach for Exploring Urinary Metabolome Modifications after Cocoa Consumption
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DOI：
10.1021/pr900470a
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发表时间：
2009-11-01
影响因子：
4.4
通讯作者：
Andres-Lacueva, Cristina
中科院分区：
文献类型：
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作者：
Llorach, Rafael;Urpi-Sarda, Mireia;Andres-Lacueva, Cristina
可可植物化学物质与可可消费的健康益处有关，代谢组学已被提议作为表征膳食组分的摄入和对代谢的影响的有力工具。在一项随机、交叉和对照试验中探索了单次摄入可可后的人尿液代谢组学改变。禁食过夜后，10名受试者随机摄入单剂量可可粉加牛奶或水，或不含可可的牛奶。在摄入前和试验餐摄入后0-6、6-12和12-24小时收集尿液样本。通过HPLC-q-ToF分析样品，然后进行多变量数据分析。结果显示，在摄入可可粉后24小时内，对尿代谢组有重要影响。这些变化不受基质的影响，因为在可可粉与牛奶或水一起食用之间没有发现全球差异。总体而言，27种代谢产物与可可-植物化学物质有关，包括生物碱衍生物、多酚代谢产物（宿主和微生物代谢物）和加工衍生的产物如二酮哌嗪，这些结果证实了代谢组学将有助于更好地表征尿液代谢组学，以进一步探索植物化学物质的代谢及其与人类健康的关系。
Cocoa-phytochemicals have been related to the health-benefits of cocoa consumption Metabolomics has been proposed as a powerful tool to characterize both the intake and the effects on the metabolism of dietary components. Human urine metabolome modifications after single cocoa intake were explored in a randomized, crossed, and controlled trial After overnight fasting, 10 subjects consumed randomly either a single dose of cocoa powder with milk or water, or milk without cocoa Urine samples were collected before the ingestion and at 0-6, 6-12, and 12-24-h after test-meals consumption. Samples were analyzed by HPLC-q-ToF, followed by multivariate data analysis. Results revealed an important effect on urinary metabolome during the 24 h after cocoa powder intake. These changes were not influenced by matrix as no global differences were found between cocoa powder consumption with milk or with water. Overall, 27 metabolites related to cocoa-phytochemicals, including alkaloid derivatives, polyphenol metabolites (both host and microbial metabolites) and processing-derived products such as diketopiperazines, were identified as the main contributors to the urinary modifications after cocoa powder intake These results confirm that metabolomics will contribute to better characterization of the urinary metabolome in order to further explore the metabolism of phytochemicals and its relation with human health.