Analysis of the expression of transglutaminases in the reconstructed human epidermis using a three-dimensional cell culture
Analysis of the expression of transglutaminases in the reconstructed human epidermis using a three-dimensional cell culture
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使用三维细胞培养物分析重建人表皮中转谷氨酰胺酶的表达
DOI：
10.1016/j.ab.2020.113606
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发表时间：
2020
影响因子：
2.9
通讯作者：
Hitomi Kiyotaka
中科院分区：
文献类型：
--
作者：
Teshima Hirofumi;Kato Manami;Tatsukawa Hideki;Hitomi Kiyotaka
皮肤表皮对各种外部应力起屏障作用。在最外层，终末分化的角质形成细胞通过在质膜下形成角质层包膜而导致具有坚韧结构的角质化。为了完成皮质包膜的形成，几种结构蛋白通过转氨酶（TG 1、TG 3、TG 5和TG 6）的催化作用交联。这些酶的表达和活化在角质形成细胞分化期间以紧密协调的方式调节。我们在这里展示了在角质形成细胞的三维培养系统中使用特异性谷氨酰胺供体底物肽检测TGases活性的系统。在这篇综述中，我们总结了表皮酶的作用，并介绍了一种检测方法，将提供一个系统的评估皮肤屏障功能。
The skin epidermis functions as a barrier to various external stresses. In the outermost layer, the terminally differentiated keratinocytes result in cornification with a tough structure by formation of a cornified envelope beneath the plasma membrane. To complete the formation of the cornified envelope, several structural proteins are cross-linked via the catalytic action of transglutaminases (TG1, TG3, TG5, and TG6). The expression and activation of these enzymes are regulated in a tightly coordinated manner during keratinocyte differentiation. We here show the system detecting the activity of the TGases using specific glutamine-donor substrate peptides in a three-dimensional culture system of keratinocytes. In this review, we summarize the roles of the epidermal enzymes and introduce a detection method that will provide a system for evaluating the skin barrier function.