Establishment and implications of a characterization method for magnetic nanoparticle using cell tracking velocimetry and magnetic susceptibility modified solutions
Establishment and implications of a characterization method for magnetic nanoparticle using cell tracking velocimetry and magnetic susceptibility modified solutions
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DOI：
10.1039/b412723d
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发表时间：
2005-01-01
期刊：
影响因子：
4.2
通讯作者：
Chalmers, JJ
中科院分区：
文献类型：
--
作者：
Zhang, HD;Moore, LR;Chalmers, JJ
与亲和标记物结合的磁性微米颗粒和纳米颗粒已经成为重要的商业试剂。已经证明，使用磁性标记的细胞分离系统的性能是可以通过标记产生的磁力的大小的函数。该磁力与结合到细胞的磁性粒子的数量、磁能梯度和粒子-场相互作用参数成比例。该粒子-场相互作用参数是微米或纳米颗粒的相对体积、磁化率和体积的乘积，是可用于表征磁性粒子的基本参数。本文介绍了一种利用磁化率修正溶液和细胞示踪测速仪CTV测量颗粒体积磁化率的实验技术。对单独的聚苯乙烯微球和与四种不同的磁性纳米颗粒结合的聚苯乙烯微球进行了实验研究。实验测定的聚苯乙烯微球的磁化率值与文献中发现的值一致。因此，结合使用商业动态光散射仪器的粒径测量的磁性纳米粒子结合这些聚苯乙烯微球的磁化率测量允许估计的粒子场相互作用参数，以四个商业，磁性纳米粒子。MACS（TM）珠的数值接近于一项独立研究报告的数值。发现MACS（TM）珠和Imag（TM）珠的值与实验观察到的一致。最后，在这个领域的相互作用参数的差异对人类淋巴细胞的标记的影响的实验演示。
Magnetic micro and nanoparticles conjugated to affinity labels have become a significant, commercial reagent. It has been demonstrated that the performance of cell separation systems using magnetic labels is a function of the magnitude of the magnetic force that can be generated through labeling. This magnetic force is proportional to the number of magnetic particles bound to the cell, the magnetic energy gradient, and the particle-field interaction parameter. This particle-field interaction parameter, which is the product of the relative volumetric, magnetic susceptibility and the volume of the micro or nanoparticle, is a fundamental parameter which can be used to characterize the magnetic particles. An experimental technique is presented which measures the volumetric magnetic susceptibility of particles through the use of susceptibility modified solutions and an experimental instrument, Cell Tracking Velocimetry, CTV. Experimental studies were conducted on polystyrene microspheres alone and those bound to four different magnetic nanoparticles. The experimentally determined values of the magnetic susceptibility of the polystyrene microspheres are consistent with values found from literature. Consequently, magnetic susceptibility measurements of these polystyrene microspheres bound with the magnetic nanoparticles combined with particle size measurements using commercial dynamic light scattering instrument allowed estimates of the particle-field interaction parameter to be made for four commercial, magnetic nanoparticles. The value found for MACS (TM) beads is close to what is reported from an independent study. The values for MACS (TM) beads and Imag (TM) beads are found to agree with what is observed from experiments. Finally, an experimental demonstration of the impact that differences in this field interaction parameter has on the labeling of human lymphocytes is presented.