NIST lipidomics workflow questionnaire: an assessment of community-wide methodologies and perspectives
NIST lipidomics workflow questionnaire: an assessment of community-wide methodologies and perspectives
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DOI：
10.1007/s11306-018-1340-1
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发表时间：
2018-05-01
期刊：
影响因子：
3.6
通讯作者：
Yost, Richard A.
中科院分区：
文献类型：
--
作者：
Bowden, John A.;Ulmer, Candice Z.;Yost, Richard A.
介绍协调脂肪组学方法的努力在社区内是有限的。在这里，我们的目的是利用最近的国家标准和技术研究所的脂肪组学实验室间比较活动，通过实施一份问卷，评估当前的方法，定量策略，标准操作程序(SOP)，以及脂肪组学社区使用的质量控制活动。目前，还没有经过社区审查的方法来评估测量的可比性或数据质量。因此，这份问卷的一个主要动机是概述当前的努力，突出需要的领域，并制定未来的目标，努力协调脂肪组学的工作流程。方法54个问题的调查询问了实验室人口统计学、脂肪组学方法和标准操作规程、分析平台、定量、参考资料、质量控制程序以及对社区内存在的挑战的看法。对结果的广泛概述强调了当前脂肪组学工作流程中广泛的方法学多样性。这种多样性对脂类测量和定量的影响目前尚不清楚，需要进一步探讨。虽然一些实验室确实纳入了标准操作规程和质量控制活动，但这些概念并没有完全被社会接受。脂肪组学领域最大的五大挑战是方法/方案之间缺乏标准化，缺乏脂类标准，缺乏软件/数据处理和量化，以及过度报告/假阳性。结论该问卷概述了当前的脂肪组学方法，并进一步促进了社区接受的指南和协议的必要性。调查问卷还作为一个平台，帮助确定要调查的计量问题并确定其优先次序。
Introduction Efforts to harmonize lipidomic methodologies have been limited within the community. Here, we aimed to capitalize on the recent National Institute of Standards and Technology lipidomics interlaboratory comparison exercise by implementing a questionnaire that assessed current methodologies, quantitation strategies, standard operating procedures (SOPs), and quality control activities employed by the lipidomics community.Objectives Lipidomics is a rapidly developing field with diverse applications. At present, there are no community-vetted methods to assess measurement comparability or data quality. Thus, a major impetus of this questionnaire was to profile current efforts, highlight areas of need, and establish future objectives in an effort to harmonize lipidomics workflows.Methods The 54-question survey inquired about laboratory demographics, lipidomic methodologies and SOPs, analytical platforms, quantitation, reference materials, quality control procedures, and opinions regarding challenges existing within the community.Results A total of 125 laboratories participated in the questionnaire. A broad overview of results highlighted a wide methodological diversity within current lipidomic workflows. The impact of this diversity on lipid measurement and quantitation is currently unknown and needs to be explored further. While some laboratories do incorporate SOPs and quality control activities, these concepts have not been fully embraced by the community. The top five perceived challenges within the lipidomics community were a lack of standardization amongst methods/protocols, lack of lipid standards, software/data handling and quantification, and over-reporting/false positives.Conclusion The questionnaire provided an overview of current lipidomics methodologies and further promoted the need for community-accepted guidelines and protocols. The questionnaire also served as a platform to help determine and prioritize metrological issues to be investigated.