Platforms to test the Temporospatial capabilities of carrier Systems in Delivering Growth Factors to benefit vascular bioengineering.
Platforms to test the Temporospatial capabilities of carrier Systems in Delivering Growth Factors to benefit vascular bioengineering.
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DOI：
10.1016/j.nano.2021.102419
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发表时间：
2021-06
期刊：
影响因子：
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通讯作者：
Lissenya B. Argueta;Jean A. Niles;Jason Sakamoto;Xuewu Liu;S. Vega;Luba Frank;Marco Paessler;J. Cor
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文献类型：
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作者：
Lissenya B. Argueta;Jean A. Niles;Jason Sakamoto;Xuewu Liu;S. Vega;Luba Frank;Marco Paessler;J. Cor
在这项研究中，我们建立了一套体外培养平台，以模拟血管细胞对生长因子和因子传递载体的反应。其中两个系统（全血管和全肺血管发育）由促进介质循环和废物清除的微流体系统支持。我们评估了Pluronic F-127水凝胶、30 nm孔径的微粒（MP）、60 nm孔径的MP或30和60 nm孔径的MP的50/50混合物的血管内皮生长因子（VEGF）递送。将VEGF递送到猪脱细胞肺血管支架（2.5cm 2的正方形片或脱细胞血管的整个3D片段）以及整个脱细胞肺支架。检查支架细胞附着以及血管组织形成。我们发现，30和60 nm孔径的硅片MP的50/50混合物允许VEGF在支架血管系统内长期释放，并在体外培养期间支持血管内皮组织发育。
In this study we produced a set ofin vitroculture platforms to model vascular cell responses to growth factors and factor delivery vehicles. Two of the systems (whole vessel and whole lung vascular development) were supported by microfluidic systems facilitating media circulation and waste removal. We assessed vascular endothelial growth factor (VEGF) delivery by Pluronic F-127 hydrogel, 30 nm pore-sized microparticles (MPs), 60 nm pore-sized MP or a 50/50 mixture of 30 and 60 nm pore-sized MP. VEGF was delivered to porcine acellular lung vascular scaffolds (2.5 cm2square pieces or whole 3D segments of acellular blood vessels) as well as whole acellular lung scaffolds. Scaffold-cell attachment was examined as was vascular tissue formation. We showed that a 50/50 mixture of 30 and 60 nm pore-sized silicon wafer MPs allowed for long-term release of VEGF within the scaffold vasculature and supported vascular endothelial tissue development duringin vitroculture.