Applicability of API ZYM to capture seasonal and spatial variabilities in lake and river sediments
Applicability of API ZYM to capture seasonal and spatial variabilities in lake and river sediments
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DOI：
10.1080/09593330.2018.1468492
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发表时间：
2019-10
影响因子：
2.8
通讯作者：
Drashti Patel;Renee Gismondi;Ali H. Alsaffar;Sonia M. Tiquia-Arashiro
中科院分区：
文献类型：
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作者：
Drashti Patel;Renee Gismondi;Ali H. Alsaffar;Sonia M. Tiquia-Arashiro
排入湖泊的水携带着大量的悬浮物，这些悬浮物是由当地流域的河流和溪流携带的。沉积物中的有机质加工是由异养微生物群落完成的，其活动具有时空差异。因此，为了捕捉和评估沉积物中的一些变化，我们在2016年春季、夏季、秋季和冬季对六个地点进行了采样：三个来自圣克莱尔河，三个来自圣克莱尔湖。在所有地点和日期，我们使用API ZYM研究了19种细胞外酶活性的时空变化。我们的结果表明，在沉积物中发现了多种活性酶。磷酸酶、脂肪酶和酯酶主要由沉积物微生物群落合成。在湖泊和沉积物样本之间没有发现一致的差异。地点和季节之间的差异更为明显。代谢（酶）多样性最高的地点反映了沉积物微生物群落分解更广泛底物的能力，可能与河流和湖泊水质的差异有关。酶活性的季节变化受人为和陆地输入引起的环境因子变化的支配，为更好地了解河湖生态系统沉积物有机质的动态提供了信息。实验结果表明，API ZYM是一种简单、快速的评价生态系统自然过程及其变化的酶分析方法。图形抽象
ABSTRACT Waters draining into a lake carry with them much of the suspended sediment that is transported by rivers and streams from the local drainage basin. The organic matter processing in the sediments is executed by heterotrophic microbial communities, whose activities may vary spatially and temporally. Thus, to capture and evaluate some of these variabilities in the sediments, we sampled six sites: three from the St. Clair River and three from Lake St. Clair in spring, summer, fall, and winter of 2016. At all sites and dates, we investigated the spatial and temporal variations in 19 extracellular enzyme activities using API ZYM. Our results indicated that a broad range of enzymes were found to be active in the sediments. Phosphatases, lipases, and esterases were synthesized most intensively by the sediment microbial communities. No consistent difference was found between the lake and sediment samples. Differences were more obvious between sites and seasons. Sites with the highest metabolic (enzyme) diversity reflected the capacity of the sediment microbial communities to breakdown a broader range of substrates and may be linked to differences in river and lake water quality. The seasonal variability of the enzymes activities was governed by the variations of environmental factors caused by anthropogenic and terrestrial inputs, and provides information for a better understanding of the dynamics of sediment organic matter of the river and lake ecosystems. The experimental results suggest that API ZYM is a simple and rapid enzyme assay procedure to evaluate natural processes in ecosystems and their changes. GRAPHICAL ABSTRACT