Identification and molecular characterisation of DNA damaging agent induced expression of Plasmodium falciparum recombination protein PfRad51
Identification and molecular characterisation of DNA damaging agent induced expression of Plasmodium falciparum recombination protein PfRad51
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DOI：
10.1016/s0020-7519(03)00212-1
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发表时间：
2003-10-01
影响因子：
4
通讯作者：
Kumar, N
中科院分区：
文献类型：
--
作者：
Bhattacharyya, MK;Kumar, N
Rad 51蛋白是大肠杆菌RecA蛋白的真核同源蛋白，在重组修复机制中起着关键作用。我们已经确定了一个新的同源Rad 51从apicomplexan寄生虫恶性疟原虫，指定PfRad 51。PfRad 51基因编码351个氨基酸的多肽，预测分子量为38720，并且在催化区域内与来自人、酵母和其它原生动物寄生虫如锥虫属和利什曼原虫属的Rad 51共享66-75%的序列同一性。在体外培养的黄疟原虫红细胞内，PfRad 51转录本和蛋白质的表达在DNA损伤剂甲磺酸甲酯的作用下上调，提示其在重组修复过程中起重要作用。PfRad 51是第一个被鉴定为编码重组蛋白的顶复门基因，它为研究此类寄生虫中DNA损伤诱导的基因表达提供了一个很好的模型。(C)2003年澳大利亚寄生虫学会由爱思唯尔有限公司出版。保留所有权利。
Rad51 protein, the eukaryotic homologue of Escherichia coli RecA protein plays a pivotal role in recombinational repair mechanism. We have identified a new homologue of Rad51 from the apicomplexan parasite Plasmodium falciparum, designated PfRad51. The PfRad51 gene codes for a 351 amino acid polypeptide with a predicted molecular mass of 38720, and shares 66-75% sequence identity within the catalytic region with Rad51 from human, yeast and other protozoan parasites such as Trypanosoma and Leishmania. The expression of PfRad51 transcript as well as protein in the intra-erythrocytic in vitro culture of P. flalciparum was found to be up-regulated in response to the DNA damaging agent methyl methanesulfonate, suggesting its functional involvement in recombinational repair process. PfRad51 is the first apicomplexan gene identified that codes for a recombination protein, and it offers an excellent model for studying DNA damage inducible gene expression in such parasites. (C) 2003 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.