Knockdown of HNRNPA1 inhibits lung adenocarcinoma cell proliferation through cell cycle arrest at G0/G1 phase
Knockdown of HNRNPA1 inhibits lung adenocarcinoma cell proliferation through cell cycle arrest at G0/G1 phase
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HNRNPA1 的敲低通过细胞周期停滞在 G0/G1 期抑制肺腺癌细胞增殖
DOI：
10.1016/j.gene.2015.11.009
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发表时间：
2016-02-01
期刊：
影响因子：
3.5
通讯作者：
Zhong, Hua
中科院分区：
文献类型：
--
作者：
Liu, Xianxun;Zhou, Yan;Zhong, Hua
异质性核核糖核蛋白A1（HNRNPA 1）是哺乳动物细胞中存在的异质性核核糖核蛋白家族成员，参与多种RNA相关过程。HNRNPA 1可促进核因子κ B α抑制亚基（I kappa B α）的降解，延长端粒。近年来，有报道称HNRNPA 1在多种肿瘤中异常表达。在本研究中，我们发现HNRNPA 1蛋白在肺癌组织中过表达。为了探讨HNRNPA 1在肺癌中的确切作用，我们利用RNA干扰（RNAi）技术对A549肺癌细胞中的HNRNPA 1进行了功能缺失分析。结果表明，HNRNPA 1基因的敲除抑制了肺癌细胞的生长和集落形成，并使细胞周期阻滞于G 0/G1期。本研究提示HNRNPA 1在肺腺癌细胞中可能发挥重要作用，为进一步研究HNRNPA 1在肺癌治疗中的作用奠定了基础。(C)2015 Elsevier B. V.版权所有。
Heterogeneous nuclear ribonucleoprotein A1 (HNRNPA1), a member of heterogeneous nuclear ribonucleoprotein family in actively growing mammalian cells, is involved in a variety of RNA-related processes. HNRNPA1 can enhance the degradation of inhibitory subunit of nuclear factor kappa B alpha (I kappa B alpha) and lengthen the telomeres. Recently, it is reported that HNRNPA1 is aberrantly expressed in varied tumors. In this study we found HNRNPA1 protein overexpressed in lung cancer tissues. To explore the exact role of HNRNPA1 in lung cancers, we carried out a loss of function analysis of HNRNPA1 in A549 lung cancer cells by RNA interference (RNAi). The results demonstrated that knockdown of HNRNPA1 inhibited cell viability and colony formation of lung cancer cells and arrested cell cycle in G0/G1 phase. Our study suggested that HNRNPA1 might play an important role in lung adenocarcinoma cells and provided a foundation for further study into the potential of HNRNPA1 for lung cancer therapy. (C) 2015 Elsevier B.V. All rights reserved.