Construction of a hierarchical micro & nanoporous surface for loading genistein on the composite of polyetheretherketone/tantalum pentoxide possessing antibacterial activity and accelerated osteointegration
Construction of a hierarchical micro & nanoporous surface for loading genistein on the composite of polyetheretherketone/tantalum pentoxide possessing antibacterial activity and accelerated osteointegration
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构建微观层次结构
DOI：
10.1039/d0bm01306d
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发表时间：
2021-01-07
影响因子：
6.6
通讯作者：
Wei, Jie
中科院分区：
文献类型：
--
作者：
Mei, Shiqi;Wang, Fan;Wei, Jie
合成了孔径约为10 nm的纳米多孔五氧化二钽（NTP）颗粒，并将其与聚醚醚酮（PEEK）共混制备了PEEK/NTP复合材料（PN）。随后，用浓硫酸处理PN以在磺化PN（SPN）上产生微孔表面（孔径约为2 μ m），这形成了分级的微米和纳米孔表面。与PN相比，SPN的多孔表面具有更高的粗糙度、亲水性和表面能。此外，染料木素（GT）被负载到SPN（SPNG）的多孔表面，显示出高的GT负载量和GT在磷酸盐缓冲液（PBS）中的缓释性。结果表明，SPNG具有良好的抑菌活性。coli和革兰氏阳性菌S.与PN相比，SPN可显著促进骨髓间充质干细胞的黏附、增殖和向成骨细胞分化。此外，与SPN相比，SPNG进一步增强了细胞应答。与PN相比，SPN能显著促进体内骨形成和骨整合。与SPN相比，SPNG进一步促进了骨整合。总之，SPNG表面具有微米和纳米多孔结构，表面带有SO 3 H基团，并能缓释GT，具有抗菌活性和促进骨整合的作用，有望作为骨替代材料的载药植入物。
Nanoporous tantalum pentoxide (NTP) particles with a pore size of about 10 nm were synthesized and blended with polyetheretherketone (PEEK) to fabricate a PEEK/NTP composite (PN). Subsequently, PN was treated by concentrated sulfuric acid to create a microporous surface (pore size of around 2 mu m) on sulfonated PN (SPN), which formed a hierarchical micro & nanoporous surface. Compared with PN, the porous surface of SPN exhibited higher roughness, hydrophilicity, and surface energy. In addition, genistein (GT) was loaded into the porous surface of SPN (SPNG), which showed high GT loading capacity and sustained release of GT into phosphate buffered saline (PBS). Moreover, SPNG revealed excellent antibacterial activity, which inhibited bacterial (E. coli and S. aureus) growth in vitro due to the synergistic effects of both sulfonic acid (SO3H) groups and the sustained release of GT. Compared with PN, SPN significantly improved the adhesion, proliferation, and osteogenic differentiation of bone mesenchymal stem cells in vitro. Moreover, compared with SPN, SPNG further enhances the cell responses. Compared with PN, SPN remarkably improved bone formation and osteointegration in vivo. Furthermore, compared with SPN, SPNG further enhanced the osteointegration. In short, SPNG with a micro & nanoporous surface, SO3H groups, and the sustained release of GT exhibited antibacterial activity and accelerated osteointegration, which would have tremendous potential as drug-loaded implants for bone substitute.