In vitro study of intestinal transport of arsenite, monomethylarsonous acid, and dimethylarsinous acid by Caco-2 cell line
In vitro study of intestinal transport of arsenite, monomethylarsonous acid, and dimethylarsinous acid by Caco-2 cell line
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DOI：
10.1016/j.toxlet.2011.04.023
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发表时间：
2011-07-28
影响因子：
3.5
通讯作者：
Velez, D.
中科院分区：
文献类型：
--
作者：
Calatayud, M.;Devesa, V.;Velez, D.
砷是一种广泛分布于环境中的污染物。关于三价砷As(III)、MMA(III)和DMA(III)的毒性研究很多，但关于这些砷的消化和吸收过程的数据很少，涉及的机制尚不清楚。本研究以Caco-2细胞模型为系统，评价了三价砷在肠道中的吸收过程。As(III)在根尖-基底外侧方向的表观渗透率为(4.6+/-0.3)×10(-6)cm/S，表现为中度的肠道吸收。甲基丙烯酸甲酯[P(APP)=(7.0+/-0.9)×10(-6)cm/S]和甲基丙烯酸甲酯[P(APP)=(10.6+/-1.4)×10(-6)cm/S]的迁移大于砷(III)。As(III)的细胞存留率(0.9-2.4%)低于MMA(III)(30%)和DMA(III)(35%)。As(III)和MMA(III)的转运过程中观察到大量的胞外成分，而DMA(III)不使用这一途径进行吸收。对于所有三价物种，运输依赖于温度，MMA(III)和DMA(III)具有跨细胞活性成分。PH值的变化不会影响这些物种的运输。GSH和绿茶提取物的存在显著改变了As(III)通过Caco-2细胞的运输。(C)2011爱思唯尔爱尔兰有限公司。保留所有权利。
Arsenic is a pollutant widely distributed in the environment. There are numerous studies on the toxicity of trivalent arsenic forms As(III), MMA(III), and DMA(III), but few data are available on the processes of digestion and absorption of these arsenic species and the mechanisms involved are unknown. The present study evaluated the processes involved in intestinal absorption of trivalent arsenic species, using the Caco-2 cell model as system. The apparent permeability values obtained for As(III) in apical-basolateral direction (4.6 +/- 0.3) x 10(-6) cm/s, showing moderate intestinal absorption. Transport of MMA(III) [P(app) = (7.0 +/- 0.9) x 10(-6) cm/s] and DMA(III) [P(app) = (10.6 +/- 1.4) x 10(-6) cm/s] is greater than that of As(III). The cellular retention of As(III) (0.9-2.4%) was less than that observed for MMA(III) (30%) and DMA(III) (35%).A substantial paracellular component was observed in transport of As(III) and MMA(III), whereas DMA(III) does not use this pathway for its absorption. For all the trivalent species, transport depends on temperature, with an active transcellular component for MMA(III) and DMA(III). Variations in pH do not affect transport of these species. The presence of GSH and green tea extract significantly alters transport of As(III) across Caco-2 cells. (C) 2011 Elsevier Ireland Ltd. All rights reserved.