P21-activated kinase 1 and 4 were associated with colorectal cancer metastasis and infiltration
P21-activated kinase 1 and 4 were associated with colorectal cancer metastasis and infiltration
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DOI：
10.1016/j.jss.2015.02.035
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发表时间：
2015-06-01
影响因子：
2.2
通讯作者：
Xu, Zhongfa
中科院分区：
文献类型：
--
作者：
Song, Bao;Wang, Wei;Xu, Zhongfa
背景：p21激活的蛋白激酶(PAK)是一种三磷酸鸟苷小效应子，在细胞的许多基本功能中发挥重要作用，包括细胞骨架重组和细胞运动。PAK在多种组织中广泛表达，在多种癌症类型中经常过度表达。方法：应用TaqMan实时定量聚合酶链式反应和免疫组织化学方法检测PAK1和PAK4在结直肠癌组织中的表达，并结合临床分析。结果：PAK1和PAK4基因在结直肠癌组织中的相对表达水平显著高于正常组织(P<0.01)。在同一癌组织中，PAK4的表达高于PAK1。随着临床分期的增加，PAK1和PAK4在癌组织中的表达逐渐增强(P<；0.01)。PAK1在淋巴结阳性患者中表达较高，PAK4在浆膜层浸润性患者中表达较高(P<；0.05)。PAK1高表达组的复发转移率高于PAK1低表达组。随访分析显示，PAK1高表达组的中位无进展生存期明显短于PAK1低表达组。结论：PAK1和PAK4的表达与结直肠癌的转移和浸润性有关，PAK1高表达提示结直肠癌预后不良。(C)2015 Elsevier Inc.保留所有权利。
Background: P21-activated kinases (PAKs) are small guanosine triphosphate effectors that play critical roles in many fundamental cellular functions, including cytoskeletal reorganization and cell motility. PAKs are widely expressed in a variety of tissues and are often overexpressed in multiple cancer types. The aim of this study was to investigate the relationship between PAK1 and PAK4 and clinicopathologic features of colorectal cancer.Methods: PAK1 and PAK4 expression in colorectal cancer patients were investigated via TaqMan real-time polymerase chain reaction and immunohistochemistry and clinical analysis.Results: The relative expression levels of PAK1 and PAK4 gene in colorectal carcinoma tissues were significantly higher than those in normal tissues (P < 0.01). PAK4 expression was higher than PAK1 in the same cancer tissue. The expression of PAK1 and PAK4 increased gradually with the clinical stages in carcinoma tissues (P < 0.01). PAK1 expression was higher in lymph node positive patients, and PAK4 expression was higher in infiltration into serous layer patients (P < 0.05). PAK1 overexpression group has a higher recurrence/metastasis rate compared with that of the PAK1 low expression group. Followup analysis showed that the median progression-free survival time of the PAK1 high expression group was significantly shorter than that of the PAK1 low expression group.Conclusions: PAK1 and PAK4 expression were associated with colorectal cancer metastasis and infiltration, PAK1 high expression may indicate poor prognosis of colorectal cancer. (C) 2015 Elsevier Inc. All rights reserved.