Determination of amatoxins in different tissues and development stages of Amanita exitialis
Determination of amatoxins in different tissues and development stages of Amanita exitialis
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鹅膏不同组织和发育阶段中鹅膏毒素的测定
DOI：
10.1002/jsfa.5685
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发表时间：
2012-10-01
影响因子：
4.1
通讯作者：
Chen, Zuohong
中科院分区：
文献类型：
--
作者：
Hu, Jinsong;Zhang, Ping;Chen, Zuohong
背景：蘑菇中毒是我国食物中毒死亡的主要原因。大多数致命的蘑菇属于鹅膏属，其鹅膏毒素是导致人类死亡的原因。鹅膏是一种常见于广东省的致死性白色蘑菇。本文研究了鹅膏蕈毒素在不同组织和发育阶段中的含量和分布。系统分析了紧急情况。结果：鹅膏毒素在六种不同蘑菇组织中的含量和分布。通过反相高效液相色谱法分析紧急情况。鹅膏毒素在鳃和菌盖中的含量最高，其次是菌柄和菌环，而在菌托和孢子中的含量最低。对不同发育阶段的蘑菇进一步分析表明，鹅膏毒素含量在蘑菇发育早期较高且稳定，在子实体生长旺盛期达到高峰，进入成熟期后急剧下降。此外，α-鹅膏蕈碱/β-鹅膏蕈碱比率在不同组织中显著变化，但在整个发育过程中在特定组织内保持恒定。结论：鹅膏蕈毒素在不同组织和发育阶段的含量和分布不同。exitialis明显不同。α-鹅膏蕈碱和β-鹅膏蕈碱在不同组织中的分布不同，但在整个发育过程中保持恒定。版权所有（C）2012化学工业协会
BACKGROUND: Mushroom poisoning is the main cause of human death by food poisoning in China. Most lethal mushrooms belong to the Amanita genus, whose amatoxins are responsible for the death of humans. Amanita exitialis is a lethal white mushroom commonly found in Guangdong Province, China. In this study the contents and distribution of the major amatoxins in different tissues and development stages of A. exitialis were systematically analysed. RESULTS: The amatoxin contents and distribution in six different mushroom tissues of A. exitialis were analysed by reverse phase high-performance liquid chromatography. The highest concentrations of amatoxins were found in the gills and pileus, followed by the stipe and annulus, with the lowest concentrations in the volva and spores. Further analysis of mushrooms in different development stages showed that the amatoxin content was relatively high and steady during early development, reached its peak when the fruit body was in the vigorous growth stage and then decreased sharply when the mushroom entered its mature stage. Furthermore, the a-amanitin/beta-amanitin ratio varied significantly in different tissues but remained constant within a specific tissue throughout development. CONCLUSION: The contents and distribution of amatoxins in different tissues and development stages of A. exitialis are markedly different. The distribution of a-amanitin and beta-amanitin varies in different tissues but remains constant throughout development. Copyright (C) 2012 Society of Chemical Industry