Feasibility and acceptability of frequent vaginal self-sampling at home by Rwandan women at high risk of urogenital tract infections.
Feasibility and acceptability of frequent vaginal self-sampling at home by Rwandan women at high risk of urogenital tract infections.
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泌尿生殖道感染高危卢旺达妇女在家中频繁进行阴道自我采样的可行性和可接受性。
DOI：
10.1136/sextrans-2020-054816
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发表时间：
2022
影响因子：
3.6
通讯作者：
Verwijs MC
中科院分区：
文献类型：
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作者：
Verwijs MC
目的：为了建立阴道微生物群（VMB）数据和临床事件之间的时间联系，需要频繁的纵向阴道采样。在受试者家中自行采集拭子可能有助于避免研究诊所和受试者负担过重。一次性阴道自我采样性病或宫颈癌筛查计划已被证明是可行的，可以接受的妇女在多项研究中，包括在撒哈拉以南非洲地区，但频繁的纵向阴道采样的背景下VMB测序studies is unknown.MethodsTwelve参与者的随机临床试验在基加利，卢旺达，自我收集阴道拭子每周三次，一个月。我们通过比较自我采集拭子与临床医生采集拭子的DNA浓度、16 S rRNA扩增子测序读数>1000的样本比例和VMB组成结果，研究了可行性。我们评估了自我收集的可接受性，使用结构化的面对面访谈和焦点小组discussion.ResultsThe参与者收集阴道拭子在131个不同的时间点。一名妇女在一次尝试后由于社会危害而停止了自我采样。所有自采样拭子均生成>1000个rRNA扩增子测序读数，自采样拭子和临床医生采样拭子的DNA浓度无显著差异（Kruskal-Wallis p=0.484）。自采样和临床医生采样拭子生成的VMB组成数据相似。参与者报告在自我采样过程中感觉非常舒适（11/12; 91.7%），并且随着时间的推移，自我采样变得越来越容易（12/12; 100%）。他们提到减少旅行时间和旅行费用的优势，自我采样在home. ConclusionsFrequently纵向阴道采样在家里是可行的，可以接受的参与者，即使在低资源的背景下，只要提供足够的咨询。
ObjectivesTo establish temporal links between vaginal microbiota (VMB) data and incident clinical events, frequent longitudinal vaginal sampling is required. Self-collection of swabs at the participant’s home may be useful to avoid overburdening research clinics and participants. One-off vaginal self-sampling for STI or cervical cancer screening programmes has been shown to be feasible and acceptable to women in multiple studies, including in sub-Saharan Africa, but the feasibility and acceptability of frequent longitudinal vaginal sampling in the context of VMB sequencing studies is unknown.MethodsTwelve participants of a randomised clinical trial in Kigali, Rwanda, self-collected vaginal swabs three times a week for a month. We studied feasibility by comparing DNA concentrations, proportions of samples with >1000 16S rRNA amplicon sequencing reads and VMB composition outcomes of self-collected swabs with clinician-collected swabs. We evaluated the acceptability of self-collection using structured face-to-face interviews and a focus group discussion.ResultsThe participants collected vaginal swabs at 131 different time points. One woman stopped self-sampling after one try due to a social harm. All self-sampled swabs generated >1000 rRNA amplicon sequencing reads, and the DNA concentration of self-sampled swabs and clinician-sampled swabs did not differ significantly (Kruskal-Wallis p=0.484). Self-sampled and clinician-sampled swabs generated similar VMB composition data. Participants reported feeling very comfortable during self-sampling (11/12; 91.7%) and that self-sampling had become easier over time (12/12; 100%). They mentioned reduced travel time and travel costs as advantages of self-sampling at home.ConclusionsFrequent longitudinal vaginal sampling at home is feasible and acceptable to participants, even in the context of a low-resource setting, as long as adequate counselling is provided.Trial registration numberNCT02459665.