Functional interleukin-7/interleukin-7Ralpha, and SDF-1alpha/CXCR4 are expressed by human periodontal ligament derived mesenchymal stem cells.
Functional interleukin-7/interleukin-7Ralpha, and SDF-1alpha/CXCR4 are expressed by human periodontal ligament derived mesenchymal stem cells.
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功能性白细胞介素 7/白细胞介素 7Rα 和 SDF-1α/CXCR4 由人牙周膜来源的间充质干细胞表达。
DOI：
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发表时间：
2008
影响因子：
5.6
通讯作者：
R. Paganelli
中科院分区：
文献类型：
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作者：
O. Trubiani;A. Isgrò;N. Zini;I. Antonucci;F. Aiuti;R. Di Primio;A. Nanci;S. Caputi;R. Paganelli
骨髓（BM）中的造血通过造血和非造血基质细胞之间的特异性相互作用来维持，所述造血和非造血基质细胞是能够产生几种细胞类型的间充质干细胞（MSC）。人牙周膜（PDL），外胚间充质起源的组织，已被证明也是MSC的来源。我们已经研究了从健康志愿者的PDL扩增的MSC是否表达与BM衍生的干细胞相似的特征，使用结构，免疫细胞化学和分子方法。还分析了它们支持造血祖细胞生长的能力。PDL-MSCs呈成纤维细胞样形态，染色质分散，基因转录活跃。间充质相关抗原CD 90，CD 29，CD 166，CD 105，和CD 44通过细胞荧光分析均匀检测，而膜CXCR 4仅由少数细胞表达。PDL-MSC在体外分化为成骨细胞和成脂细胞。IL-7、IL-7 R α、SDF-1 α和CXCR 4的免疫定位导致弥散但特异性的标记。RT-PCR分析证实了上述转录本的表达。这些细胞自发地产生高水平的IL-7和SDF-1 α，并且能够比鼠基质细胞更有效地支持BM前体细胞的发育和长期维持，并且与正常BM人基质细胞相似。我们检测了成脂和成骨分化过程中IL-7和SDF-1 α的分泌途径。IL-7在成骨和成脂分化过程中增加，而SDF-1 α分泌在成骨分化过程中下调，但在成脂诱导过程中增加。我们的研究提供的证据表明，在人类PDL中，有一个可访问的MSC生态位，显示出BM衍生的MSC的特征。
Hematopoiesis in the bone marrow (BM) is maintained by specific interactions between both hematopoietic and non-hematopoietic stromal cells, which are mesenchymal stem cells (MSCs) capable of giving rise to several cell types. The human periodontal ligament (PDL), a tissue of ectomesenchymal origin, has been shown to also be a source of MSCs. We have investigated whether MSCs expanded from the PDL of healthy volunteers express characteristics similar to BM-derived stem cells using structural, immunocytochemical and molecular approaches. Their ability to support the growth of hematopoietic progenitors was also analyzed. The PDL-MSCs exhibited a fibroblast-like morphology and their chromatin was dispersed, indicating active gene transcription. The mesenchymal-related antigens CD90, CD29, CD166, CD105, and CD44 were homogeneously detected by cytofluorimetric analysis, whereas membrane CXCR4 was expressed only by a minority of cells. The PDL-MSCs differentiated in vitro into osteogenic and adipogenic cells. Immunolocalization of IL-7, IL-7Ralpha, SDF-1alpha, and CXCR4 resulted in a diffuse but specific labeling. RT-PCR analysis confirmed the expression of the above-mentioned transcripts. The cells spontaneously produced high levels of IL-7 and SDF-1alpha and were able to support the development and long-term maintenance of BM precursor cells more efficiently than murine stromal cells and similarly to normal BM human stromal cells. We examined IL-7 and SDF-1alpha secretion pathway during adipogenic and osteogenic differentiation. IL-7 increased during osteogenic and adipogenic differentiation, while the SDF-1alpha secretion was downregulated during osteogenic differentiation but increased during adipogenic induction. Our study provides evidence that in human PDL there is an accessible niche of MSCs showing the features of BM-derived MSCs.