DEVELOPMENT AND TISSUE-SPECIFIC DISTRIBUTION OF MOUSE SMALL HEAT-SHOCK PROTEIN HSP25
DEVELOPMENT AND TISSUE-SPECIFIC DISTRIBUTION OF MOUSE SMALL HEAT-SHOCK PROTEIN HSP25
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DOI：
10.1002/dvg.1020140204
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发表时间：
1993-01-01
期刊：
影响因子：
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通讯作者：
KLOETZEL, PM
中科院分区：
文献类型：
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作者：
GERNOLD, M;KNAUF, U;KLOETZEL, PM
我们已经研究了小鼠小热休克蛋白25的免疫组织学的发展和组织特异性分布，使用抗体，特异性识别热休克蛋白25。我们的分析表明，热休克蛋白25的相对量增加胚胎发生过程中。在胚胎发生的第13-20天，hsp 25主要在各种肌肉组织中积累，包括心脏、膀胱和背部肌肉。在脊髓神经元和浦肯野细胞中也可检测到HSP 25。此外，对密切相关的α，B-晶状体蛋白的分析表明，在几种组织中，包括膀胱、脊索鞘和眼透镜，两种蛋白质共表达。我们的研究表明，哺乳动物热休克蛋白25的积累在小鼠胚胎发育过程中受到发育调控，并支持小的热休克蛋白在正常细胞代谢中的重要功能作用的观点。
We have investigated the developmental and tissue-specific distribution of the mouse small hsp25 by immunohistology using an antibody that specifically identifies hsp25. Our analysis shows that the relative amount of hsp25 increases during embryogenesis. Through days 13-20 of embryogenesis, hsp25 accumulation is predominant in the various muscle tissues, including the heart, the bladder, and the back muscles. hsp25 is detectable also in neurons of the spinal cord and the purkinje cells. Furthermore analysis of the closely related alpha, B-crystallin shows that in several tissues, including the bladder, the notochordal sheath and the eye lens both proteins are coexpressed. Our studies demonstrate that mammalian hsp25 accumulation is developmentally regulated during mouse embryogenesis and support the view of an important functional role of small heat shock proteins in normal cell metabolism.