EPR and affinity studies of mannose-TEMPO functionalized PAMAM dendrimers.
EPR and affinity studies of mannose-TEMPO functionalized PAMAM dendrimers.
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甘露糖-TEMPO 功能化 PAMAM 树枝状聚合物的 EPR 和亲和力研究。
DOI：
10.1039/b411643g
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发表时间：
2004
影响因子：
3.2
通讯作者：
Cloninger,MaryJ
中科院分区：
文献类型：
--
作者：
Samuelson,LynnE;Sebby,KarlB;Walter,EricD;Singel,DavidJ;Cloninger,MaryJ
合成了随甘露糖含量增加的甘露糖-TEMPO功能化G(4)-PAMAM树状大分子，并用MALDI-TOF、MS和EPR光谱进行了表征。通过分析这些树枝状大分子的EPR谱中的线宽效应，我们可以确定甘露糖和TEMPO在树枝状大分子表面的相对呈现。用血凝试验和亲和层析/EPR实验评价了甘露糖-TEMPO树枝状大分子与刀豆蛋白A的结合活性。
Mannose–TEMPO functionalized G(4)-PAMAM dendrimers with increasing mannose loadings have been synthesized and characterized by MALDI-TOF MS and EPR spectroscopy. Analysis of linebroadening effects in the EPR spectra of these dendrimers allowed us to determine the relative presentation of mannose and TEMPO on the dendrimer surface. Hemagglutination assays and affinity chromatography/EPR experiments to assess the activity of the mannose–TEMPO dendrimers with Concanavalin A are presented.