Electrophysiological characteristics of classes of neuron in the HVc of the zebra finch
Electrophysiological characteristics of classes of neuron in the HVc of the zebra finch
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DOI：
10.1152/jn.1998.80.2.914
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发表时间：
1998-08-01
影响因子：
2.5
通讯作者：
Taniguchi, I
中科院分区：
文献类型：
--
作者：
Kubota, M;Taniguchi, I
全细胞记录是由斑胸草雀HVc神经元在切片制备中进行的。根据它们的电生理特性，发现了四种不同的神经元。还通过细胞内注射荧光黄检查了其中一些神经元的形态学特征。I型神经元（65个细胞中的21个）在动作电位后的后超极化达到峰值的时间比其他类别长。他们表现出快速和石灰依赖性内向整流和一个初始的高频率发射，然后是一个较慢的恒定发射。I型神经元胞体大，树突厚，棘多。这类神经元的轴突向嗅旁叶X区方向投射。II型神经元（65个细胞中的30个）具有比其他类别更负的静息膜电位。他们表现出快速的内在矫正。II型神经元可分为两个亚类的情况下（IIa; 22细胞）和存在（IIb; 8细胞）的低阈值瞬时去极化。aa型神经元胞体相对较小，树突细而多刺。这类神经元中的一些神经元的轴突投射在原始纹状体（RA）的健壮核的方向上。IIb型神经元胞体较大，树突较粗，棘较多。nr型神经元（65个细胞中有6个）的动作电位持续时间比其他类型的短。他们表现出突出的时间依赖性内向整流和定期紧张性发射很少或没有住宿。III型神经元有串珠状、多刺的树突。TV型神经元（65个细胞中的8个）具有较长的动作电位持续时间：比其他类更大的输入电阻和更长的膜时间常数。IV型神经元胞体小，树突薄而短，稀疏多刺。这类神经元中的一些神经元的轴突向RA的方向投射。这些类别的神经元可能在HVc的发声和表达中发挥不同的作用。
Whole cell recordings were made from zebra finch HVc neurons in slice preparations. Four distinct classes of neuron were found on the basis of their electro-physiological properties. The morphological characteristics of some of these neurons were also examined by intracellular injection of Lucifer yellow. Type I neurons (21 of 65 cells) had longer time-to-peak of an afterhyperpolarization following an action potential than the other classes. They exhibited both fast and lime-dependent inward rectification and an initial high-frequency firing followed by a slower constant firing. Type I neurons had large somata and thick dendrites with many spines. The axons of some of the neurons in this class projected in the direction of area X of the parolfactory lobe. Type II neurons (30 of 65 cells) had a more negative resting membrane potential than the other classes. They exhibited fast inward rectification. Type II neurons could be divided into two subclasses by the absence (IIa; 22 cells) and the presence (IIb; 8 cells) of a low-threshold transient depolarization. Type aa neurons had relatively small somata and thin, spiny dendrites. The axons of some of the neurons in this class projected in the direction of the robust nucleus of the archistriatum (RA). Type IIb neurons had relatively large somata and thick dendrites with many spines. Type nr neurons (6 of 65 cells) had a shorter action-potential duration than the other classes. They exhibited prominent time-dependent inward rectification and a regular tonic firing with little or no accommodation. Type III neurons had beaded, aspiny dendrites. Type TV neurons ( 8 of 65 cells) had a longer action-potential duration: a much larger input resistance, and longer membrane time constant than the other classes. Type IV neurons had small somata and thin, short, sparsely spiny dendrites. The axons of some of the neurons in this class projected in the direction of the RA. These classes of neuron may play distinct roles in song production and representation in the HVc.