Adaptive Liquid Interfacially Assembled Protein Nanosheets for Guiding Mesenchymal Stem Cell Fate
Adaptive Liquid Interfacially Assembled Protein Nanosheets for Guiding Mesenchymal Stem Cell Fate
复制标题
DOI：
10.1002/adma.201905942
复制
发表时间：
2019-12-09
影响因子：
29.4
通讯作者：
Ariga, Katsuhiko
中科院分区：
文献类型：
--
作者：
Jia, Xiaofang;Minami, Kosuke;Ariga, Katsuhiko
动态自适应生物材料的发展对细胞功能的调控越来越感兴趣。然而，现有的材料仅限于呈现和去除细胞粘附生物活性基序的双态切换，其不能在体内模拟具有连续可调特性的天然细胞外基质（ECM）。在这里，可调的自适应材料组成的蛋白质单层组装在液-液界面，动态适应细胞牵引力。通过界面堵塞，发生从蛋白质单层到分层纤维的超微结构转变。拉长的纤连蛋白纤维促进拉长的粘着斑结构的形成，增加粘着斑激酶的活化，并增强干细胞的神经元分化。细胞牵引力导致ECM蛋白的空间重排，其反馈以改变干细胞命运。报道的仿生自适应液体界面能够动态控制干细胞的行为，并具有潜在的翻译应用。
There is a growing interest in the development of dynamic adaptive biomaterials for regulation of cellular functions. However, existing materials are limited to two-state switching of the presentation and removal of cell-adhesive bioactive motifs that cannot emulate the native extracellular matrix (ECM) in vivo with continuously adjustable characteristics. Here, tunable adaptive materials composed of a protein monolayer assembled at a liquid-liquid interface are demonstrated, which adapt dynamically to cell traction forces. An ultrastructure transition from protein monolayer to hierarchical fiber occurs through interfacial jamming. Elongated fibronectin fibers promote formation of elongated focal adhesion structures, increase focal adhesion kinase activation, and enhance neuronal differentiation of stem cells. Cell traction force results in spatial rearrangement of ECM proteins, which feeds back to alter stem cell fate. The reported biomimetic adaptive liquid interface enables dynamic control of stem cell behavior and has potential translational applications.