Continuous-flow sorting of microalgae cells based on lipid content by high frequency dielectrophoresis
Continuous-flow sorting of microalgae cells based on lipid content by high frequency dielectrophoresis
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DOI：
10.3934/biophy.2016.3.398
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发表时间：
2016-01-01
期刊：
影响因子：
1.5
通讯作者：
Geiger, Emil J.
中科院分区：
文献类型：
--
作者：
Hadady, Hanieh;Redelman, Doug;Geiger, Emil J.
本文提出了一种连续流动的细胞筛选装置，分离和分离微藻细胞（衣藻）的基础上，脂质含量的高频（50 MHz）介电电泳。由于横向DEP力相对于水动力之间的平衡，该装置能够筛选微藻。正DEP力沿着施加在流过平面叉指电极的细胞上的调幅电场，以Z字形图案操纵低脂质细胞轨迹。理论建模证实了分选过程中的细胞轨迹。采用时程实验进行分离定量和灵敏度分析，并对采集的样品进行流式细胞仪分析。在不同的时间段进行了氮饥饿dw 15 -1（高脂，HL）和pgd 1突变株（低脂，LL）的实验测试，并实现了两个群体的明确分离。实验结果表明，氮饥饿过程中产生了三个种群：HL、LL和低叶绿素（LC）种群。LC群体的存在会影响二元分离性能。在氮饥饿的第6天，使用50 MHz、30伏峰-峰AC电场，连续流动微分离器可以从起始样品中分离出74%的HL和75%的LL。在氮饥饿的第9天，LL（低脂质：在出口#1处为86.1%）和LC（在出口#2处为88.8%）之间发生分离。该设备可用于现场监测;因此，它有可能降低生物燃料的生产成本。
This paper presents a continuous-flow cell screening device to isolate and separate microalgae cells (Chlamydomonas reinhardtii) based on lipid content using high frequency (50 MHz) dielectrophoresis. This device enables screening of microalgae due to the balance between lateral DEP forces relative to hydrodynamic forces. Positive DEP force along with amplitude-modulated electric field exerted on the cells flowing over the planar interdigitated electrodes, manipulated low-lipid cell trajectories in a zigzag pattern. Theoretical modelling confirmed cell trajectories during sorting. Separation quantification and sensitivity analysis were conducted with time-course experiments and collected samples were analysed by flow cytometry. Experimental testing with nitrogen starved dw15-1 (high-lipid, HL) and pgd1 mutant (low-lipid, LL) strains were carried out at different time periods, and clear separation of the two populations was achieved. Experimental results demonstrated that three populations were produced during nitrogen starvation: HL, LL and low-chlorophyll (LC) populations. Presence of the LC population can affect the binary separation performance. The continuous-flow micro-separator can separate 74% of the HL and 75% of the LL out of the starting sample using a 50 MHz, 30 voltages peak-to-peak AC electric field at Day 6 of the nitrogen starvation. The separation occurred between LL (low-lipid: 86.1% at Outlet # 1) and LC (88.8% at Outlet # 2) at Day 9 of the nitrogen starvation. This device can be used for onsite monitoring; therefore, it has the potential to reduce biofuel production costs.