The biophysical effects of localized electrochemical therapy on porcine skin
The biophysical effects of localized electrochemical therapy on porcine skin
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DOI：
10.1016/j.jdermsci.2020.01.006
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发表时间：
2020-03-01
影响因子：
4.6
通讯作者：
Wong, Brian J. F.
中科院分区：
文献类型：
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作者：
Pham, Tiffany T.;Hong, Ellen M.;Wong, Brian J. F.
背景：微创方法治疗疤痕解决了改变胶原结构的共同途径，导致胶原重塑。目的：在本研究中，我们采用原位氧化还原化学在皮肤中产生局部pH梯度，改变真皮胶原蛋白，这一过程被我们称为电化学治疗（ECT）。研究了电刺激对离体猪皮肤生化和结构变化的影响。方法：电痉挛时，在新鲜猪皮上插入两个铂电极，注射生理盐水后施加电位。采用pH作图、高频超声、双光子激发显微镜和二次谐波显微镜（SHG）评价治疗效果。结果与组织学相关。结果：在ECT之后，pH图分别描述了阳极和阴极的酸和碱的产生，随着电压和施加时间的增加。超声观察电痉挛过程中气体形成情况。阳极显示明显的SHG信号缺失，而阴极显示胶原蛋白结构紊乱，可获得的信号原纤维较少。组织学上证实两个部位的胶原变性。结论：我们通过ECT证实了皮肤中酸和碱的原位产生。这种作用通过化学和精确地改变变性的胶原结构，使人们认识到ECT作为一种简单、低成本、微创的皮肤重塑和疤痕治疗手段的潜力。(C) 2020年日本皮肤病研究学会。Elsevier B.V.版权所有。
Background: Minimally-invasive methods to treat scars address a common pathway of altering collagen structure, leading to collagen remodeling.Objective: In this study, we employed in situ redox chemistry to create focal pH gradients in skin, altering dermal collagen, in a process we refer to as electrochemical therapy (ECT). The effects of ECT to induce biochemical and structural changes in ex vivo porcine skin were examined.Methods: During ECT, two platinum electrodes were inserted into fresh porcine skin, and following saline injection, an electrical potential was applied. pH mapping, high frequency ultrasonography, and two photon excitation microscopy and second harmonic generation (SHG) microscopy were used to evaluate treatment effects. Findings were correlated with histology.Results: Following ECT, pH mapping depicted acid and base production at anode and cathode sites respectively, with increasing voltage and application time. Gas formation during ECT was observed with ultrasonography. Anode sites showed significant loss of SHG signal, while cathode sites showed disorganized collagen structure with fewer fibrils emitting an attainable signal. Histologically, collagen denaturation at both sites was confirmed.Conclusion: We demonstrated the production of in situ acid and base in skin occurring via ECT. The effects chemically and precisely alter collagen structure through denaturation, giving insight on the potential of ECT as a simple, low-cost, and minimally-invasive means to remodel skin and treat scars. (C) 2020 Japanese Society for Investigative Dermatology. Published by Elsevier B.V. All rights reserved.