A conserved mechanism of retrovirus restriction in mammals
A conserved mechanism of retrovirus restriction in mammals
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DOI：
10.1073/pnas.200286297
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发表时间：
2000-10-24
影响因子：
11.1
通讯作者：
Danos, O
中科院分区：
文献类型：
--
作者：
Towers, G;Bock, M;Danos, O
鼠Fv 1基因限制N-或B-嗜性鼠白血病病毒在植入后、整合前阶段的感染。先前用于研究Fv 1的Fv 1敏感病毒编码亲嗜性包膜基因，因此仅感染啮齿动物细胞。因此，仅在小鼠和鼠细胞系中进行了Fv 1限制性研究。通过用含有N-或B-嗜性核心和泛嗜性水泡性口炎病毒-G包膜蛋白的逆转录病毒载体感染，我们现在证明来自各种哺乳动物物种（包括人）的细胞系具有Fv 1样逆转录病毒限制性功能，防止N-嗜性载体感染。与Fv 1一样，限制性内切酶直接作用于病毒衣壳蛋白的第110位氨基酸。与Fv 1相反，新的限制的特点是不存在逆转录病毒DNA，我们推测这些活动已被选定的逆转录病毒流行病在遥远的过去。
The murine Fv1 gene restricts infection by N- or B-tropic murine leukemia viruses at a postentry, preintegration stage. The Fv1-sensitive viruses previously used for the study of Fv1 encode an ecotropic envelope gene and thus only infect rodent cells. Consequently, the study of Fv1 restriction has been carried out solely in mice and murine cell lines. By infection with retroviral Vectors containing N- or B-tropic core and pantropic vesicular stomatitis virus-G envelope protein, we now demonstrate that cell lines derived from various mammalian species, including humans, have an Fv1-like retrovirus restriction function, preventing N-tropic vector infection. Like Fv1, restriction is directed at amino acid 110 of the viral capsid protein. In contrast to Fv1, the novel restriction is characterized by the absence of reverse-transcribed viral DNA, We speculate that these activities have been selected for by retroviral epidemics in the distant past.