Differential distribution of subchains of the basement membrane components type IV collagen and laminin among the amniotic membrane, cornea, and conjunctiva
Differential distribution of subchains of the basement membrane components type IV collagen and laminin among the amniotic membrane, cornea, and conjunctiva
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DOI：
10.1097/00003226-199901000-00013
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发表时间：
1999-01-01
期刊：
影响因子：
2.8
通讯作者：
Nishida, T
中科院分区：
文献类型：
--
作者：
Fukuda, K;Chikama, T;Nishida, T
目的。据报道，羊膜移植是重建眼前节的有效手术方法。为了更好地了解移植羊膜的功能，我们将羊膜中TV型胶原和层粘连蛋白的子链分布与角膜和结膜中的分布进行了比较。方法，将五片人角膜及结膜和三片人羊膜冷冻在OCT复合物中。用低温恒温器切割冷冻切片并通过间接免疫荧光显微镜染色。我们使用了胶原蛋白 α 2(IV) 和 α 5(IV) 子链、层粘连蛋白-1、层粘连蛋白-5、纤连蛋白和 VII 型胶原蛋白的抗体。结果，在结膜基底膜和羊膜中，胶原蛋白α2(IV)荧光明显，而胶原蛋白α5(IV)荧光不明显。相比之下，在角膜基底膜中，胶原蛋白 α 5 (IV) 子链的荧光明显，但胶原蛋白 α 2 (IV) 子链的荧光不明显。所有检查的基底膜中均存在层粘连蛋白-1、层粘连蛋白-5、纤连蛋白和VII型胶原。结论。羊膜中IV型胶原α亚链的分布与结膜中的分布相同，但与角膜中的分布不同。没有观察到其他基底膜成分的分布模式存在差异。这些结果表明，羊膜和结膜的基底膜可能具有相同的成分；因此，羊膜可能可用作结膜基底膜的替代品。
Purpose. Amniotic membrane transplantation has been reported to be an effective surgical procedure for the reconstruction of the anterior segment of the eye. To understand better the function of transplanted amniotic membrane, we compared the distributions of subchains of type TV collagen and laminin in the amniotic membrane to those in the cornea and conjunctiva. Methods, Five human corneas with conjunctivas and three human amniotic membranes were frozen in OCT compound. Cryosections were cut with a cryostat and stained by an indirect immunofluorescent microscopy. We used antibodies of the collagen alpha 2(IV) and alpha 5(IV) subchains, laminin-1, laminin-5, fibronectin, and type VII collagen. Results, In the conjunctival basement membrane and the amniotic membrane, fluorescence was evident for collagen alpha 2(IV) but not for collagen alpha 5(IV). By contrast, in the corneal basement membrane, fluorescence was apparent for the collagen alpha 5(IV) subchain but not for the collagen alpha 2(IV) subchain. Laminin-1, laminin-5, fibronectin, and type VII collagen were present in all the basement membranes examined. Conclusion. The distribution of alpha subchains of type IV collagen in the amniotic membrane was identical to that in the conjunctiva but different from that in the cornea. No difference in the distribution pattern of other basement membrane components was observed. These results demonstrate that the basement membrane of the amniotic membrane and the conjunctiva might share the same components; therefore, the amniotic membrane might be useful as a replacement for the basement membrane of the conjunctiva.