Use of a thermal lens microscope in integrated catecholamine determination on a microchip
Use of a thermal lens microscope in integrated catecholamine determination on a microchip
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使用热透镜显微镜在微芯片上集成儿茶酚胺测定
DOI：
10.1007/s002160100998
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
T. Kitamori
中科院分区：
文献类型：
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作者：
Hussein M. Sorouraddin;A. Hibara;T. Kitamori
利用微芯片技术和热透镜显微镜，建立了一种测定儿茶酚胺的新方法。采用250 μm×100 μm的微通道进行混合、反应和检测。采用偏高碘酸钠氧化氨基色素显色法测定肾上腺素（EP）、去甲肾上腺素（NE）、多巴胺（DA）和左旋多巴（LD）。使用热透镜显微镜（TLM）检测产物。该系统的灵敏度与四种CA相当，样品和试剂混合仅需15 s。校准线表明，浓度为5-20 μg mL-1时具有良好的线性。10 μg mL-1溶液中EP、NE、DA和LD的相对标准偏差分别为1.08、2.18、2.2和2.5%。用该系统测定了药物注射剂中的CA，结果与标称值相关良好。通过使用该集成系统获得的结果表明，有足够的可能性实现高通量的医疗诊断系统。
A new method for determination for catecholamines (CA) utilizing microchip technology and a thermal lens microscope has been developed. Microchannels with a 250 μm×100 μm cross section were used for mixing, reaction, and detection. Epinephrine (EP), nor-epinephrine (NE), dopamine (DA), andL-dopa (LD) were determined by using coloring oxidization to aminochromes by sodium metaperiodate. A thermal lens microscope (TLM) was used for detection of the product. The sensitivity of the system was comparable for the four CA and required only 15 s for mixing of sample and reagent. The calibration lines indicated excellent linearity for concentrations of 5–20 μg mL–1. The relative standard deviations for 10 μg mL–1solution were 1.08, 2.18, 2.2, and 2.5% for EP, NE, DA, and LD, respectively. CA in pharmaceutical injections were also determined by use of the system and the results correlated very well with nominal values. Results obtained by use of the integrated system suggested there was a sufficient possibility to realize high-throughput medical diagnosis systems.