Reproductive toxicities of ofloxacin and norfloxacin on Caenorhabditis elegans with multi-generational oscillatory effects and trans-generational residual influences.
Reproductive toxicities of ofloxacin and norfloxacin on Caenorhabditis elegans with multi-generational oscillatory effects and trans-generational residual influences.
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DOI：
10.1016/j.etap.2022.103962
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发表时间：
2022-08
影响因子：
4.3
通讯作者：
Jing Zhang;Yungu Zheng;Zhenyang Yu
中科院分区：
文献类型：
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作者：
Jing Zhang;Yungu Zheng;Zhenyang Yu
世代生殖毒性是评价氟喹诺酮类抗生素长期环境影响的重要指标。本文研究了氧氟沙星（OFL）和诺氟沙星（NOR）对秀丽隐杆线虫连续9代（F1-F9）生殖的影响。结果表明，OFL对F1无作用，对F2 ~ F4有促进作用，对F5 ~ F9有抑制作用。NOR的效应也表现出跨代的刺激和抑制之间的振荡。进一步的生化分析表明，OFL和NOR的生殖毒性与总胆固醇（TCHO）、孕酮（P）和睾酮（T）的关系比与精子主要蛋白（MSP）和卵黄蛋白原（Vn）的关系更密切。此外，OFL和NOR在T4和F1和F9的曾孙T4′中也表现出明显的跨代生殖毒性。T4与T4′、OFL与NOR之间的差异提示了多代暴露的影响，提示进一步探讨不同荧光质之间的差异机制。
The reproductive toxicities over generations are essential to assess the long-term impacts environmental fluoroquinolone antibiotics (FQs). In the present study, the multi-generational effects of ofloxacin (OFL) and norfloxacin (NOR) on reproduction were studied on Caenorhabditis elegans from 9 successive generations (F1-F9). Results showed that OFL showed no effects in F1, stimulation in F2 to F4, and inhibition F5 to F9. The effects of NOR also showed oscillation between stimulation and inhibition across generations. Further biochemical analysis demonstrated that the reproductive toxicities of OFL and NOR were more closely connected with total cholesterol (TCHO), progesterone (P) and testosterone (T), than major sperm protein (MSP) and vitellogenin (Vn). Moreover, OFL and NOR also showed significant trans-generational reproductive toxicities in T4 and T4′, the great-grand-daughter of F1 and F9. Differences between T4 and T4′ and between OFL and NOR, indicated influences of multi-generational exposure and urged more exploration on different mechanisms between FQs.