Polar growth in the Alphaproteobacterial order Rhizobiales
Polar growth in the Alphaproteobacterial order Rhizobiales
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DOI：
10.1073/pnas.1114476109
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发表时间：
2012-01-31
影响因子：
11.1
通讯作者：
Brun, Yves V.
中科院分区：
文献类型：
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作者：
Brown, Pamela J. B.;de Pedro, Miguel A.;Brun, Yves V.
许多杆状细菌的伸长通过肽聚糖合成发生在沿着细胞侧壁沿着的离散焦点处。然而，在根瘤菌目中，有许多出芽细菌，其中新细胞的生长被限制在特定区域。根瘤菌目的共生性表明这种带状生长方式可能是祖先的。我们证明，杆状细菌根癌农杆菌生长单向从细胞分裂后产生的新的极，并具有非典型的肽聚糖组合物。极性生长发生在所有测试条件下，包括当细胞附着在植物根上时和在诱导毒力的条件下。最后，我们表明，极性生长也发生在密切相关的细菌苜蓿中华根瘤菌，布鲁氏菌，和Ochrobactrum anthropi。我们发现，单极生长是一个祖先和保守的特性之间的根瘤菌，其中包括重要的互惠生和病原体的植物和动物。
Elongation of many rod-shaped bacteria occurs by peptidoglycan synthesis at discrete foci along the sidewall of the cells. However, within the Rhizobiales, there are many budding bacteria, in which new cell growth is constrained to a specific region. The phylogeny of the Rhizobiales indicates that this mode of zonal growth may be ancestral. We demonstrate that the rod-shaped bacterium Agrobacterium tumefaciens grows unidirectionally from the new pole generated after cell division and has an atypical peptidoglycan composition. Polar growth occurs under all conditions tested, including when cells are attached to a plant root and under conditions that induce virulence. Finally, we show that polar growth also occurs in the closely related bacteria Sinorhizobium meliloti, Brucella abortus, and Ochrobactrum anthropi. We find that unipolar growth is an ancestral and conserved trait among the Rhizobiales, which includes important mutualists and pathogens of plants and animals.