Functional expression of ERG1 potassium channels in rat alveolar macrophages
Functional expression of ERG1 potassium channels in rat alveolar macrophages
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大鼠肺泡巨噬细胞ERG1钾通道的功能表达
DOI：
10.1007/s10735-012-9458-3
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发表时间：
2013-02-01
影响因子：
3.2
通讯作者：
Dong, Mingqing
中科院分区：
文献类型：
--
作者：
Dong, Haiying;Ji, Zhenyu;Dong, Mingqing
肺泡巨噬细胞（Alveolar macrophages，AM）在肺免疫中起着重要作用。近年来的研究表明，钾通道与巨噬细胞的活化、迁移和细胞因子分泌等功能密切相关。然而，很少有人知道的表达和功能的ERG通道在AM。结果表明，ERG 1通道在大鼠肺泡巨噬细胞中有表达，且在LPS刺激后表达水平增加。E4031阻断ERG 1通道后，ERG 1蛋白的成熟度降低，NF-κB的核转位减少，LPS刺激的IL-6和IL-1β的分泌减少。这些结果表明ERG 1通道在大鼠肺泡巨噬细胞中功能性表达，并在炎症反应中发挥重要作用。
Alveolar macrophages (AMs) play a vital role in lung immunity. The recent studies demonstrated that potassium channels were associated with macrophage functions, such as activation, migration and cytokines secretion. However, less is known regarding the expression and function of ERG channels in AMs. Our study showed that ERG1 channel expressed in rat alveolar macrophage, and the expression level was increased when AMs were stimulated with LPS. Furthermore, blockade of ERG1 channels with E4031 down-regulated the mature of ERG1 protein, inhibited NF-κB translocation into the nucleus, and reduced LPS-stimulated IL-6 and IL-1β secretion. These results imply that ERG1 channels are functionally expressed in rat alveolar macrophages and play an important role in inflammatory response.