Continuous antimicrobial mechanism of dispersible hydroxyapatite nanoparticles doped with zinc ions for percutaneous device coatings
Continuous antimicrobial mechanism of dispersible hydroxyapatite nanoparticles doped with zinc ions for percutaneous device coatings
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DOI：
10.1177/08853282221108839
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发表时间：
2022-06
影响因子：
2.9
通讯作者：
Marina Oshita;Koji Umeda;Minami Kataoka;Y. Azuma;T. Furuzono
中科院分区：
文献类型：
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作者：
Marina Oshita;Koji Umeda;Minami Kataoka;Y. Azuma;T. Furuzono
经皮器械-留置导管相关感染是严重的临床事件。因此，有必要开发适用于器械的抗感染涂层材料以获得长期有效性。本课题组研制了具有抗菌活性的金属离子或卤素离子掺杂的高分散性结晶羟基磷灰石纳米粒子。采用湿化学法制备了含锌量为13.5%（Zn/（Zn + Ca）× 100）的抗菌、分散性好、结晶性好的锌掺杂羟基磷灰石（Zn（15）-HAp）纳米粒子。采用大肠杆菌（Escherichia coli，E. coli）和金黄色葡萄球菌（Staphylococcus aureus）。细菌在Zn（15）-HAp纳米颗粒表面的存活率明显低于普通HAp（nHAp）表面，但对L929细胞的增殖无影响。即使在PBS中浸泡2周后，Zn（15）-HAp纳米颗粒对E.大肠杆菌的培养液保持在与浸泡20分钟相似的水平。细菌的死亡不仅与锌、镁离子之间的离子交换现象有关，还与细胞内活性氧的积累有关。Zn（15）-HAp纳米颗粒可能不容易发生过敏样反应，因为与纳米颗粒共培养的HMC-1细胞释放的组胺量与nHAp无显著差异，但在统计学上远低于氯己定。
Percutaneous devices—indwelling catheters—related infections are serious clinical incidents. It is accordingly necessary to develop anti-infective coating materials suitable for the devices for long-term effectiveness. In our research group, highly dispersible and crystalline hydroxyapatite (HAp) nanoparticles doped with metallic or halogen ions possessing antibacterial activities have been developed. In this study, antibacterial, dispersible, and crystalline zinc (Zn)-doped hydroxyapatite [Zn(15)-HAp] nanoparticles substituted with 13.5% Zn content [Zn/(Zn + Ca) × 100] were prepared by a wet chemical method using an anti-sintering agent through calcination. Antibacterial activities of Zn(15)-HAp nanoparticles were evaluated using Escherichia coli (E. coli) and Staphylococcus aureus. The survival rates of the bacteria on Zn(15)-HAp nanoparticles were significantly lower than that on normal HAp (nHAp) coated surfaces, while no influences were observed on proliferation of L929 cells. Even after soaking Zn(15)-HAp nanoparticles in PBS for 2 weeks, the antibacterial activities against E. coli were maintained at a similar level to a 20 min soaking. The bacterial death was related to not only ion-exchange phenomenon between Zn and magnesium ions but also accumulation of reactive oxygen species (ROS) in the cells. Allergic-like reactions—anaphylactoid reactions—might not readily occur with Zn(15)-HAp nanoparticles because the amounts of histamine released from HMC-1 cells co-cultured with nanoparticles were not significantly different to that of nHAp, but were statistically much lower than that of chlorhexidine.