Susceptibility of Cherries to Bacterial Canker ( Pseudomonas syringae pv. syringae ) in Field and Laboratory
Susceptibility of Cherries to Bacterial Canker ( Pseudomonas syringae pv. syringae ) in Field and Laboratory
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樱桃在田间和实验室对细菌性溃疡病（丁香假单胞菌丁香致病变种）的敏感性
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
A. Soleimani
中科院分区：
文献类型：
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作者：
S. Farhadfar;M. Keshavarzi;N. Bouzari;L. Moghadam;A. Soleimani
细菌性溃疡病由假单胞菌pv.樱桃溃疡病是世界范围内樱桃的一种重要病害，但对樱桃细菌性溃疡病的抗病育种和抗病品种的选育重视不够。本研究采用田间和室内人工接种的方法，对21个伊朗甜樱桃品种和7个引进甜樱桃品种进行了溃疡病抗性鉴定。同时还研究了溃疡病抗性与木材直径的相关性。使用LOPAT和GATTa试验研究了三种当地的P. eriningae菌株，并将其用作接种物。实验室试验以2年生的休眠枝条为材料，接种后1个月测定溃疡长度。在田间试验中，在深秋接种2年生植株的树干和枝条，8个月后测量病斑长度。根据结果，在离体枝条中，病斑长度在Shamloo中最低，在KB 25中最高。在这两个器官在现场条件下测试，Siyah-daneshkadeh是最敏感的和Albaloo meshkinshahr最耐。聚类分析将供试品种分为高感、感病和中感3个相对感病类群，分别占供试材料的3.6%、70.7%和25%。现场和实验室数据之间没有相关性，但树器官溃疡长度相关。病变集体在树干比拍摄和拍摄直径和坏死长度之间存在直接的相关性。总之，樱桃对P.s. PV.果园条件下人工接种的方法是品种鉴别的推荐方法。
Bacterial canker caused by Pseudomonas syringae pv. syringae is an important disease in cherries worldwide but low attention has been paid to breeding and selection of cherries for bacterial canker resistance. In this research, 21 selected Iranian and 7 introduced cultivars of sweet/sour/ducke cherries were examined for resistance to bacterial canker by artificial inoculation in field and laboratory condition. Correlation between canker resistance with wood diameter was also examined. Three local P. syringae strains were studied using LOPAT and GATTa tests and used as inoculum. For laboratory test, two-years-old dormant shoots were used and the canker length measured one month after inoculation. For field assay, trunk and shoots of two-years-old plants were inoculated in late autumn and eight months later, lesion length was measured. Based on result, in excised shoots, lesion length was the lowest in Shamloo and the highest in KB25. In both organs tested in field condition, Siyah-daneshkadeh was the most susceptible and Albaloo-meshkinshahr the most resistant. Cluster analysis grouped the cultivars in three relative susceptibility groups including highly susceptible, susceptible and intermediate constituting 3.6%, 70.7%, and 25% of the material, respectively. No correlation was found between field and laboratory data but canker length in tree organs correlated together. Lesions were collectively larger in trunk than shoot and a direct correlation existed between shoot diameter and necrosis length. In conclusion, cherries vary in susceptibility to P. s. pv. syringae and artificial inoculation in orchard condition is the recommended method for cultivar discrimination.