Regulation of eukaryotic initiation factor-2 expression during sepsis.
Regulation of eukaryotic initiation factor-2 expression during sepsis.
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败血症期间真核起始因子 2 表达的调节。
DOI：
10.1152/ajpendo.1994.266.2.e193
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Kimball,SR
中科院分区：
文献类型：
--
作者：
Vary,TC;Jurasinski,CV;Karinch,AM;Kimball,SR
在无菌或脓毒性脓肿大鼠肝脏肽链起始水平上刺激蛋白质合成。相比之下，在脓毒症大鼠的快速抽搐骨骼肌中，肽链的启动受到抑制。我们通过测量真核起始因子-2 （eIF-2）的细胞含量、eIF-2 α亚基的磷酸化程度和eIF-2B的活性，研究了脓毒症期间肝脏和骨骼肌之间肽链起始差异变化的可能机制。在骨骼肌中，败血症期间eIF-2含量和eIF-2 α磷酸化程度均未改变。然而，在快速收缩肌肉中观察到eIF-2B活性显著降低（P < 0.001）。在肝脏中，无菌脓肿或脓毒性脓肿大鼠的eIF-2 α磷酸化程度和eIF-2B活性与对照组相比均无差异。然而，在无菌性炎症和败血症中，肝脏中eIF-2的含量均增加。与对照组相比，两种情况下eIF-2 α mRNA的相对丰度均未增加。对照大鼠的eIF-2 α mRNA分布分析显示，只有大约40%的信息与多聚体相关。与对照组相比，无菌炎症或败血症导致与多体相关的eIF-2 α mRNA的比例增加50%。（摘要删节250字）
Protein synthesis is stimulated at the level of peptide chain initiation in livers from rats with a sterile or septic abscess. In contrast, peptide chain initiation is inhibited in fast-twitch skeletal muscles from septic rats. We investigated the possible mechanisms responsible for these differential changes in peptide chain initiation between liver and skeletal muscle during sepsis by measuring the cellular content of eukaryotic initiation factor-2 (eIF-2), the extent of phosphorylation of the alpha-subunit of eIF-2, and the activity of eIF-2B. In skeletal muscle, neither the eIF-2 content nor the extent of phosphorylation of eIF-2 alpha was altered during sepsis. However, a significant decrease (P < 0.001) in eIF-2B activity was observed in fast-twitch muscles. In liver, neither the extent of phosphorylation of eIF-2 alpha nor the activity of eIF-2B was different in rats with a sterile or septic abscess compared with control. However, the amount of eIF-2 in liver was increased in both sterile inflammation and sepsis. The relative abundance of eIF-2 alpha mRNA was not increased in either condition compared with control. Analysis of the distribution of eIF-2 alpha mRNA from control rats revealed that only approximately 40% of the message was associated with polysomes. Sterile inflammation or sepsis caused a 50% increase in the proportion of eIF-2 alpha mRNA associated with the polysomes compared with control.(ABSTRACT TRUNCATED AT 250 WORDS)