Plasmid-mediated transformation tropism of chlamydial biovars.
Plasmid-mediated transformation tropism of chlamydial biovars.
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DOI：
10.1111/2049-632x.12104
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发表时间：
2014-03
影响因子：
3.3
通讯作者：
Caldwell HD
中科院分区：
文献类型：
--
作者：
Song L;Carlson JH;Zhou B;Virtaneva K;Whitmire WM;Sturdevant GL;Porcella SF;McClarty G;Caldwell HD
沙眼衣原体和C. muridarum分别是人类和小鼠的病原体，在基因序列和基因内容上具有高度的保守性。这两个物种都含有一个共同的7.5 kb质粒，这是一个重要的毒力因子。最近描述的转换系统已被用于表征C。沙眼衣原体L2质粒基因的功能，然而，类似的研究还没有报道C。沙眼衣原体嗜眼血清型A或小鼠株，C. muridarum。在这里，我们用C. A型和C型沙眼衣原体。（i）成功转化了C. muridarum和C.沙眼衣原体血清型A被限制于具有C. muridarum或C.沙眼衣原体血清型A质粒骨架，ii）质粒缺陷型C. muridarum与C.基于鼠伤寒沙门氏菌的穿梭载体补充了糖原积累和包涵体形态，和（iii）C.鼠伤寒沙门氏菌质粒编码的Pgp 4是染色体（glgA）和质粒（pgp 3）毒力基因的调节因子。总之，我们的研究结果显示了衣原体质粒在其转化嗜性中的一个先前未被认识和意想不到的作用，并证实了衣原体毒力基因的质粒调节作用。muridarum。
Chlamydia trachomatis and C. muridarum are human and mouse pathogens respectively, which show high conservation of gene order and content. Both species contain a common 7.5 kb plasmid that is an important virulence factor. Recently described transformation systems have been used to characterize C. trachomatis L2 plasmid gene functions; however, similar studies have not been reported for C. trachomatis ocular tropic serovar A or the mouse strain, C. muridarum. Here, we have conducted genetic experiments with C. trachomatis serovar A and C. muridarum and report the following; (i) successful transformation of C. muridarum and C. trachomatis serovar A is restricted to a shuttle vector with a C. muridarum, or C. trachomatis serovar A plasmid backbone, respectively, ii) transformation of plasmid-deficient C. muridarum with the C. muridarum based shuttle vector complemented glycogen accumulation and inclusion morphology, and (iii) C. muridarum plasmid encoded Pgp4 is a regulator of chromosomal (glgA) and plasmid (pgp3) virulence genes. In summary, our findings show a previously unrecognized and unexpected role for the chlamydial plasmid in its transformation tropism and confirm the plasmids regulatory role of virulence genes in C. muridarum.