Quantitative genetic interaction mapping using the E-MAP approach.
Quantitative genetic interaction mapping using the E-MAP approach.
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DOI：
10.1016/s0076-6879(10)70009-4
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发表时间：
2010
影响因子：
--
通讯作者：
Krogan, Nevan J.
中科院分区：
文献类型：
--
作者：
Collins, Sean R.;Roguev, Assen;Krogan, Nevan J.
遗传相互作用表示一个突变的存在调节第二个突变的表型的程度。近年来，系统和定量地测量遗传相互作用的方法已被证明是无偏表征基因功能的有效工具，并为进化分析提供了有价值的数据。在这里，我们提出了两种酵母物种的生物体生长速率的遗传相互作用的系统测量协议。
Genetic interactions represent the degree to which the presence of one mutation modulates the phenotype of a second mutation. In recent years, approaches for measuring genetic interactions systematically and quantitatively have proven to be effective tools for unbiased characterization of gene function and have provided valuable data for analyses of evolution. Here, we present protocols for systematic measurement of genetic interactions with respect to organismal growth rate for two yeast species.