Preparation of Hemocompatible Poly(lactic-co-glycolic acid)-F127 Nanospheres and Their Application to Biosensor for Analysis of Whole Blood.
Preparation of Hemocompatible Poly(lactic-co-glycolic acid)-F127 Nanospheres and Their Application to Biosensor for Analysis of Whole Blood.
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DOI：
10.1166/jnn.2015.8771
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发表时间：
2015
影响因子：
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通讯作者：
Chong Sun;Y. Niu;Xu-jie Yang;Min Liu;Xiaodi Yang;Xiaohua Huang;Wenbo Zhao
中科院分区：
文献类型：
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作者：
Chong Sun;Y. Niu;Xu-jie Yang;Min Liu;Xiaodi Yang;Xiaohua Huang;Wenbo Zhao
本文合成了新型聚乳酸-羟基乙酸共聚物-F127纳米球（PLGA-F127 NSs），并将其用于构建可直接应用于全血的电流型葡萄糖生物传感器。葡萄糖生物传感器的这种性质是基于PLGA-F127 NS的抗生物污染性质。详细介绍了PLGA-F127纳米粒的制备及葡萄糖氧化酶（GOx）在PLGA-F127/玻碳电极（GCE）上的固定化。研究了该传感器在全血中的电化学行为。循环伏安法结果表明，固定在PLGA-F127 NS上的GOx表现出直接的电子转移反应，从而产生了稳定的葡萄糖电流生物传感，检测限为5.57 x 10（-6）M（S/N = 3）。葡萄糖生物传感器对血液中正常浓度的抗坏血酸（AA）和尿酸（UA）无响应。材料科学的发展将为与人类健康相关的诊断和治疗相关的高性能生物传感器带来重大投入。
In this paper, the novel poly(lactic-co-glycolic acid)-F127 nanospheres (PLGA-F127 NSs) were synthesized and used to establish an amperometric glucose biosensor that can be applied in whole blood directly. This property of glucose biosensor was based on the antibiofouling property of PLGA-F127 NSs. More details of preparing PLGA-F127 NSs and immobilizing glucose oxidase (GOx) on (PLGA-F127)/glass carbon electrode (GCE) were presented. Then, the electrochemical behaviors of the biosensor in whole blood were studied. The cyclic voltammetric results indicated that GOx immobilized on PLGA-F127 NSs exhibited direct electron transfer reaction, which led to stable amperometric biosensing for glucose with a detection limit of 5.57 x 10(-6) M (S/N = 3). The glucose biosensor did not respond to ascorbic acid (AA) and uric.acid (UA) at their concentration normally encountered in blood. The development of materials science will bring significant input to high-performance biosensors relevant to diagnostics and therapy of interest for human health.