Novel plasmid-mediated 16S rRNA methylase, RmtC, found in a Proteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides
Novel plasmid-mediated 16S rRNA methylase, RmtC, found in a Proteus mirabilis isolate demonstrating extraordinary high-level resistance against various aminoglycosides
复制标题
DOI：
10.1128/aac.50.1.178-184.2006
复制
发表时间：
2006-01-01
影响因子：
4.9
通讯作者：
Arakawa, Y
中科院分区：
文献类型：
--
作者：
Wachino, JI;Yamane, K;Arakawa, Y
奇异变形杆菌 ARS68 于 2003 年从日本一名住院患者身上分离出来，对各种氨基糖苷类药物表现出非常高水平的耐药性。通过一般的接合实验，该菌株的氨基糖苷类耐药性无法转移到受体菌株大肠杆菌CSH-2和大肠杆菌HB101上，但大肠杆菌DH5α通过亲本菌株ARS68的质粒成功电转化，并获得了异常高度的氨基糖苷类耐药性。克隆和测序分析表明，一种名为 rmtC 的新型 16S rRNA 甲基化酶基因的存在是 ARS68 菌株及其转化体产生抗性的原因。 rmtC的G+C含量为41.1%，推导的新鉴定的16S rRNA甲基化酶RmtC的氨基酸序列具有较低水平的同一性（
Proteus mirabilis ARS68, which demonstrated a very high level of resistance to various aminoglycosides, was isolated in 2003 from an inpatient in Japan. The aminoglycoside resistance of this strain could not be transferred to recipient strains Escherichia coli CSH-2 and E. coli HB101 by a general conjugation experiment, but E. coli DH5 alpha was successfully transformed by electroporation with the plasmid of the parent strain, ARS68, and acquired an unusually high degree of resistance against aminoglycosides. Cloning and sequencing analyses revealed that the presence of a novel 16S rRNA methylase gene, designated rmtC, was responsible for resistance in strain ARS68 and its transformant. The G+C content of rmtC was 41.1%, and the deduced amino acid sequences of the newly identified 16S rRNA methylase, RmtC, shared a relatively low level of identity (