Effects of hydrogen sulfide on myocardial fibrosis in diabetic rats: Changes in matrix metalloproteinases parameters.
Effects of hydrogen sulfide on myocardial fibrosis in diabetic rats: Changes in matrix metalloproteinases parameters.
复制标题
硫化氢对糖尿病大鼠心肌纤维化的影响：基质金属蛋白酶参数的变化。
DOI：
10.3233/bme-151508
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Yang, Jun
中科院分区：
文献类型：
--
作者：
Luo, Jian;Wu, Zhixiong;Li, Fang;Yang, Jun
为探讨硫化氢（H2S）对糖尿病大鼠心肌纤维化的影响及其机制，采用链脲佐菌素腹腔注射建立糖尿病大鼠模型，以硫氢化钠（NaHS）作为H2S的外源性供体。8周后，采用货车Gieson染色观察心肌病理变化，碱性水解法测定羟脯氨酸含量，Western Blot法测定MMP 2、MMP 7、MMP 11、MMP 13、MMP 16、TIMP 1、TGFβ 1的表达。结果显示，心肌纤维化明显，TIMP 1、MMP 2表达减少，MMP 7、MMP 11、MMP 13、MMP 16、TIMP 1、TGFβ 1表达增加，MMP 11、MMP 13、MMP 16、MMP 17、MMP 18、MMP 19、MMP 1 MMP 16在糖尿病组有表达，但NaHS治疗后这些变化均被明显逆转。提示H2S可减轻糖尿病大鼠心肌纤维化，其机制可能与调节MMPs/TIMPs表达及TGFβ1的表达有关。
In order to explore the effects of hydrogen sulfide (H2S) on myocardial fibrosis in diabetic rats and its underlying mechanisms, intraperitoneal injections of streptozotocin were used to establish the diabetes models and sodium hydrosulfide (NaHS) was used as an exogenous donor of H2S. Eight weeks later, Van Gieson staining was used to observe pathological changes, and basic hydrolysis methods were adopted to measure hydroxyproline content, while Western Blot was used to determine the expression of MMP2, MMP7, MMP11, MMP13, MMP16, TIMP1 and TGFβ1.The results showed that significant myocardial fibrosis, decreased TIMP1 and MMP2 expression and increased MMP7, MMP11, MMP13, MMP16 expressions occurred in diabetic group, but all these changes were significantly reversed in diabetic rats after NaHS treatment. This suggests that H2S could attenuate cardiac fibrosis induced by diabetes and its mechanisms may be related to its modulation of MMPs/TIMPs expression and regulation of TGFβ1.
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影响因子：
6
作者：
Zhou, Xiang;An, Guoyin;Lu, Xiang
通讯作者：
Lu, Xiang
影响因子：
4.3
作者：
Xin Zhong;Leihong Wang;Yuwen Wang;Shiyun Dong;Xiaoning Leng;J. Jia;Yajun Zhao;Hulun Li;
通讯作者：
Xin Zhong;Leihong Wang;Yuwen Wang;Shiyun Dong;Xiaoning Leng;J. Jia;Yajun Zhao;Hulun Li;
影响因子：
3.6
作者：
Taye, Ashraf;Abouzied, Mekky M.;Mohafez, Omar M. M.
通讯作者：
Mohafez, Omar M. M.
影响因子：
4.8
作者：
Vellaichamy, E;Khurana, ML;Pandey, KN
通讯作者：
Pandey, KN
气态硫化物可预防部分独立于低代谢的小鼠的心肌缺血 - 再灌注损伤。
影响因子：
3.7
作者：
Snijder PM;de Boer RA;Bos EM;van den Born JC;Ruifrok WP;Vreeswijk-Baudoin I;van Dijk MC;Hillebrands JL;Leuvenink HG;van Goor H
通讯作者：
van Goor H