Labeled Schwann cell transplantation: Cell loss, host Schwann cell replacement, and strategies to enhance survival
Labeled Schwann cell transplantation: Cell loss, host Schwann cell replacement, and strategies to enhance survival
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作者：
Hill, CE;Moon, LDF;Bunge, MB
尽管在脊髓损伤模型中，移植的雪旺细胞(SCs)可以促进轴突再生和再髓鞘形成，并促进恢复，但对它们的存活以及它们如何与宿主组织相互作用知之甚少。使用表达人胎盘碱性磷酸酶(PLAP)的转基因大鼠的标记干细胞，评估脊髓挫伤损伤中SC的存活。急性移植后2周仅有少量PLAP干细胞存活。他们因坏死和细胞凋亡而过早死亡。将移植推迟到损伤后7天可提高存活率。在存活的细胞整合到脊髓后，发生了第二波细胞死亡。环孢素免疫抑制可提高血小板干细胞的存活率；延迟移植联合免疫抑制的存活率最高。在所有病例中，干细胞移植导致内源性p75(+)细胞广泛渗透到损伤部位，提示内源性干细胞可能在干细胞移植后的修复中发挥重要作用。(C)2005年Wiley-Liss，Inc.
Although transplanted Schwann cells (SCs) can promote axon regeneration and remyelination and improve recovery in models of spinal cord injury, little is known about their survival and how they interact with host tissue. Using labeled SCs from transgenic rats expressing human placental alkaline phosphatase (PLAP), SC survival in a spinal cord contusion lesion was assessed. Few PLAP SCs survived at 2 weeks after acute transplantation. They died early due to necrosis and apoptosis. Delaying transplantation until 7 days after injury improved survival. A second wave of cell death occurred after surviving cells had integrated into the spinal cord. Survival of PLAT SCs was enhanced by immunosuppression with cyclosporin; delayed transplantation in conjunction with immunosuppression resulted in the best survival. In all cases, transplantation of SCs resulted in extensive infiltration of endogenous p75(+) cells into the injury site, suggesting that endogenous SCs may play an important role in the repair observed after SC transplantation. (C) 2005 Wiley-Liss, Inc.