A High-Adhesive Lysine-Cyclic RGD Peptide Designed for Selective Cell Retention Technology
A High-Adhesive Lysine-Cyclic RGD Peptide Designed for Selective Cell Retention Technology
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专为选择性细胞保留技术而设计的高粘附性赖氨酸环 RGD 肽
DOI：
10.1089/ten.tec.2015.0517
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发表时间：
2016-06-01
影响因子：
3
通讯作者：
Luo, Fei
中科院分区：
文献类型：
--
作者：
Luo, Keyu;Mei, Tieniu;Luo, Fei
细胞粘附是选择性细胞保留（SCR）技术中使用的生物材料的重要特性，SCR技术在临床环境中快速制造骨移植物。这可以通过物理和生物操作来改善。为了促进细胞在支架上的保留，特别是在方便的SCR程序中来自骨髓的骨祖细胞，本文设计了赖氨酸-环状RGD（LcRGD）肽以协调带正电荷的氨基酸和RGD序列，以增强支架的粘附性能。脱矿骨基质（DBM）是一种重要的治疗资源，但由于其加工过程，其细胞粘附能力和骨诱导能力较低。当用于SCR技术时，可以增加这些能力以增强DBM的性能。在此，LcR ⑶肽用于修饰DBM并产生DBM/LcR ⑶复合物。该复合材料对培养的人骨髓间充质干细胞表现出增强的粘附性能，并比其他材料保留了更多的骨髓骨祖细胞。DBM/LcRGD复合物具有较好的体外成骨诱导作用和体内成骨能力。因此，LcRGD肽作为一种值得称道的DBM修饰剂应用于SCR技术中可以改善骨移植。
Cell adhesion is an important property of biomaterials used in selective cell retention (SCR) technology, which fabricates bone grafts rapidly in clinical settings. This could be improved by physical and biologic manipulations. To facilitate retention of the cells on the scaffold, especially osteoprogenitors from bone marrow in the convenient SCR procedure, a lysine-cyclic RGD (LcRGD) peptide was here designed to coordinate positively charged amino acids and the RGD sequence to enhance the adhesion performance of the scaffold. Demineralized bone matrix (DBM) is an important therapeutic resource, but its cell adhesion ability and osteoinductive capacity are low because of its processing. These capabilities can be increased to enhance the performance of DBM when used in SCR technology. Here, LcRGD peptide was used to modify DBM and produce a DBM/LcRGD composite. This composite exhibited enhanced adhesion performance on cultured human bone marrow-derived mesenchymal stem cells and retained more osteoprogenitors from bone marrow than other materials did. The DBM/LcRGD composite displayed a preferable osteoinduction in vitro and osteogenic capacity in vivo. Thus, LcRGD peptide as a commendable modifier of DBM applied in SCR technology can improve bone transplantation.